Our Anti-Raf-1 (Ser642) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Raf-1 (Ser642) and is antigen affinity purified. It is great for use in WB.
Western blot of HeLa lysate showing specific labeling of the ~74 kDa Raf 1 protein phosphorylated at Ser642 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser-301 and Ser-642.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
RAF1
74 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser642 of rat Raf-1, conjugated to keyhole limpet hemocyanin (KLH).
Human, Rat
Bovine, Canine, Chicken, Mouse, Non-Human Primate
AB_2492224
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~74 kDa Raf-1 protein phosphorylated at Ser642. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser642
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Stangl, C., et al. 2020. Diverse BRAF gene fusions confer resistance to EGFR-targeted therapy via differential modulation of BRAF activity. Molecular Cancer Research, 18(4), pp.537-548.
