Our Anti-Rabphilin 3A rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Rabphilin 3A and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat cortical lysate showing specific immunolabeling of the ~82 kDa rabphilin 3A.
Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser-234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
RPH3A
82 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat rabphilin 3A conjugated to keyhole limpet hemocyanin (KLH).
Rat
Bovine, Canine, Human, Non-Human Primate, Mouse
AB_2492221
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~82 kDa rabphilin 3A protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Foletti, D.L. and Scheller, R.H., 2001. Developmental regulation and specific brain distribution of phosphorabphilin. Journal of Neuroscience, 21(15), pp.5461-5472. PMID: 11466417
Fykse, E.M., Li, C. and Sudhof, T.C., 1995. Phosphorylation of rabphilin-3A by Ca2+/calmodulin-and cAMP-dependent protein kinases in vitro. Journal of Neuroscience, 15(3), pp.2385-2395. PMID: 7891174
Geppert, M., Bolshakov, V.Y., Siegelbaum, S.A., Takei, K., De Camilli, P., Hammer, R.E. and Südhof, T.C., 1994. The role of Rab3A in neurotransmitter release. Nature, 369(6480), p.493-7. PMID: 7911226
Li, C., Takei, K., Geppert, M., Daniell, L., Stenius, K., Chapman, E.R., Jahn, R., De Camilli, P. and Südhof, T.C., 1994. Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C. Neuron, 13(4), pp.885-898. PMID: 7946335
Smith, R., Petersén, Å., Bates, G.P., Brundin, P. and Li, J.Y., 2005. Depletion of rabphilin 3A in a transgenic mouse model (R6/1) of Huntington's disease, a possible culprit in synaptic dysfunction. Neurobiology of Disease, 20(3), pp.673-684. PMID: 15967669
Related Products
Recently Viewed
Your research is our top priority
Highly-validated antibodies and reagents from our family of brands.
