Anti-Proto-oncogene tyrosine-protein kinase receptor Ret (RET) Antibody

Name: Anti-Proto-oncogene tyrosine-protein kinase receptor Ret (RET) Antibody
Brand: Biosensis
SKU: C-1663-100
Price: 457.0 USD
Availability: InStock

Our Anti-Proto-oncogene tyrosine-protein kinase receptor Ret (RET) chicken polyclonal primary antibody detects human Proto-oncogene tyrosine-protein kinase receptor Ret (RET), and is purified. It is validated for use in ICC, WB.

Immunofluorescence detection of human RET expressed in U2OS cells. RET was visualized using Chicken polyclonal antibody to human RET (dilution 1:500) and Goat anti-chicken IgY - H&L DyLight 550 (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. Composite picture was generated using pseudocolors green for RET specific signal and blue for nuclei.

Western-Blot detection of human RET expressed in CHO cells. Line 1. hRET-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 uL of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. Rabbit polyclonal to chicken IgY was used as secondary antibody.

Immunofluorescence detection of human RET expressed in U2OS cells. RET was visualized using Chicken polyclonal antibody to human RET (dilution 1:500) and Goat anti-chicken IgY - H&L DyLight 550 (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. Composite picture was generated using pseudocolors green for RET specific signal and blue for nuclei.

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Species Reactivity

Human

Applications

ICC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1663-100

Volume/Size: 100 µg

Variant

Price:

$457.00

Quantity:

Product Details

Target

Proto-oncogene tyrosine-protein kinase receptor Ret (RET)

Scientific Background

The RET proto-oncogene is a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor family of extracellular signalling molecules

Format

Purified

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, WB

Host Species

Chicken

Immunogen

Recombinant His-tagged extracellular fragment of human RET protein produced using CHO cell line. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.

Immunogen Species

Human

Species Reactivity

Human

Storage

Store antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally.Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250 mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80°C until used. Prevent multiple freeze thaw cycles of aliquots for best product life. Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.

Physical State

Liquid

Buffer

Concentrated ammonium sulphate in PBS pH 7.4

Dilution Ranges

WB: 1:5000-1:10000
ICC: 1:250-1:500

Conjugate

Unconjugated

Specificity

Human RET.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Cadherin family member 12; Ret;

UniProt ID

P07949

Shipping

2-8°C (on cold packs)

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Anti-Proto-oncogene tyrosine-protein kinase receptor Ret (RET) Antibody

SKU: C-1663-100

Product Details

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