Anti-Proteolipid Protein (PLP) Antibody

Name: Anti-Proteolipid Protein (PLP) Antibody
Brand: Aves Labs
SKU: PLP
Price: 470.0 USD
Availability: InStock

3 Citations

An antipeptide antibody was generated in chickens against a sequence shared between the mouse (P60202) and human (NP_000524) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL. These antibodies have been validated with human, mouse and rat tissues.

Immunohistochemistry photomicrograph of PLP immunoreactivity (green; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain. Blue is DAPI nuclear counterstain. Western blot shows a single band in adult mouse brain lysate, 27 ug loaded, 10% gel. Hoda Ilias, Aves Labs.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: PLP

Volume: 1000 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Proteolipid Protein (PLP)

Scientific Background

Human Proteolipid Protein (PLP, or lipophilin) is a 29,946 dalton protein (277 amino acids) found in myelin of the CNS and PNS. Expressed by oligodendrocytes and Schwann cells, PLP stabilizes myelin by preventing lipid bilayer fusion, and aids in its compaction. Different mutations of the human PLP gene product result in two neurological disorders: Pelizaeus-Merzbacher Disease and Spastic Paraplegia Type 2.

Format

Affinity-Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

PLP1 PLP

Molecular Weight

30 kDa

Immunogen

Synthetic peptide

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313560

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The sequence of the synthetic peptide was shared between the human and mouse gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentration of the eluate adjusted to 100 µg/mL, and the preparation was filter sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:2000-1:5000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Synonyms

Myelin proteolipid protein (PLP) (Lipophilin)

UniProt ID

P60201

Shipping

Ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Mouse

Immunocytochemistry: Rat
PMID	Dilution	Publication
32910475	Not Listed	Nakamura, D.S., et al. 2020. Mitochondrial Dynamics and Bioenergetics Regulated by Netrin-1 in Oligodendrocytes.. Glia, 392-412.
29251594	1:500	Lin, J.P., et al. 2017. LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair. eLife, e30498.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
30406183	1:100	Micheva, K.D., et al. 2018. Distinctive Structural and Molecular Features of Myelinated Inhibitory Axons in Human Neocortex.. eNeuro, .

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Anti-Proteolipid Protein (PLP) Antibody

SKU: PLP

Product Details

Product Specific References for Applications and Species

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