Our Anti-Progesterone Receptor (Ser190) phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Progesterone Receptor (Ser190) and is Protein G purified. It is great for use in WB, IHC.
Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser190. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).
There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser-190 and Ser-294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.
Protein G Purified
Monoclonal
1154
IgG1
IHC, WB
Mouse
PGR
90/120 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser190 of human progesterone receptor, conjugated to keyhole limpet hemocyanin (KLH).
Human
AB_2492215
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatant
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
IHC: 1:1000
Unconjugated
Specific for endogenous levels of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform phosphorylated at Ser190. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser190
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
NR3C3 antibody, Nuclear receptor subfamily 3 group C member 3 antibody, PGR antibody, PR antibody, PRA antibody, PRB antibody, PRGR_HUMAN antibody, Progesterone receptor antibody, Progestin receptor form A antibody, Progestin receptor form B antibody
Attia, G.R., Zeitoun, K., Edwards, D., Johns, A., Carr, B.R. and Bulun, S.E., 2000. Progesterone receptor isoform A but not B is expressed in endometriosis. The Journal of Clinical Endocrinology & Metabolism, 85(8), pp.2897-2902. PMID: 10946900
Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., Schrader, W.T., Weigel, N.L. and Edwards, D.P., 2000. Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody. Molecular Endocrinology, 14(1), pp.52-65.PMID: 10628747
Edwards, D.P., 2005. Regulation of signal transduction pathways by estrogen and progesterone. Annual Review ofPhysiology, 67, pp.335-376. PMID: 15709962
