Anti-pro-Brain-derived neurotrophic factor (proBDNF) Antibody

Name: Anti-pro-Brain-derived neurotrophic factor (proBDNF) Antibody
Brand: Biosensis
SKU: R-087-100
Price: 327.0 USD
Availability: InStock

Our Anti-pro-Brain-derived neurotrophic factor (proBDNF) rabbit polyclonal primary antibody detects human, mouse, other mammals (predicted), and rat pro-Brain-derived neurotrophic factor (proBDNF), and is whole serum. It is validated for use in IHC-Frozen, WB.

Immunofluorescence analysis of proBDNF expression in human SHSY-5Y cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) SHSY-5Y cells were incubated with proBDNF antibody R-087-100 (1:1000, green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-rabbit-CF488A antibody (4 µg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). BDNF-IR is observed in the perinuclear cytoplasm. Magnification: 100x.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

IHC, WB

Host Species

Rabbit

Go to Full Product Details

Data Sheet

SKU: R-087-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

pro-Brain-derived neurotrophic factor (proBDNF)

Scientific Background

BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

IHC, WB

Host Species

Rabbit

Immunogen

A synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Dilution Ranges

WB: 1:1000-1:5000
IHC: 1:1000-1:5000
ICC: 1:500-1:2000

Application Details

Antibody works on 4% formaldehyde fixed cells.

Conjugate

Unconjugated

Specificity

Used in Western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antiserum is known to react with human, mouse and rat proBDNF and also expected to recognize other mammalian proBDNF.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Brain-derived neurotrophic factor (Precursor); Abrineurin

UniProt ID

P23560

Shipping

25°C (ambient)

Recover password

Create my account

Anti-pro-Brain-derived neurotrophic factor (proBDNF) Antibody

SKU: R-087-100

Product Details

Bulk Order Anti-pro-Brain-derived neurotrophic factor (proBDNF) Antibody

Related Products

Recently Viewed