Anti-Prion Protein (Ser-43), Phosphospecific Antibody
Our Prion Protein (Ser-43) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Prion Protein (Ser-43) and is antigen affinity purified. It is great for use in WB.
SKU: PP3951
Product Details
Prion Protein (Ser-43)
Prion related neurodegenerative diseases, called transmissible spongiform encephalopathies, are observed in many animal species. These diseases involve conversion of normal cellular prion protein (PrPc) into a form that is insoluble and resistant to proteases (PrPSc). The protease resistant form can polymerize into fibrils which accumulate in infected tissues and cause cell death and tissue damage. PrPs have an N-terminal signal sequence and a C-terminal linkage to glycosylphosphatidylinositol anchor. The mature protein is a glycosylated protein that associates with cell membranes. Phosphorylation of PrPC at Ser-43 by Cdk5 promotes proteinase K resistance, prion aggregation, and fibril formation in vitro. In addition, Ser-43 phosphorylation is upregulated in scrapie-infected mouse brain relative to controls. Thus, phosphorylation of Ser-43 may be an important mechanism leading conversion of PrPc to PrPSc and the onset of disease.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
PRNP
27
Prion Protein (Ser-43) antibody was generated from a phospho-peptide that included amino acids surrounding Serine 43 in human prion protein. This sequence has high homology to the conserved site in rat, mouse, and bovine prion protein.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
Unconjugated
The antibody detects a human recombinant Prion protein after phosphorylation by Cdk5/p25 complex. In addition, the antibody may detect aggregrated forms of prion in human PC3 cells treated with Calyculin A.
Phosphorylated
Ser-43
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
PrP, PrPsc, PrPc
UniProt (Human): P04156
UniProt (Immunogen Species): P04156
UniProt (Immunogen Species): P04156