Anti-Potassium Chloride Cotransporter (KCC2) (Thr1007) Antibody
Our Anti-Potassium Chloride Cotransporter (KCC2) (Thr1007) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Potassium Chloride Cotransporter (KCC2) (Thr1007) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Thr1007 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Product Specific References for Applications and Species
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 37688574 | 1:5000 | Liao, W, et al. 2023. CDKL5-mediated developmental tuning of neuronal excitability and concomitant regulation of transcriptome. Human molecular genetics, . |
| 36973513 | not listed | Hu, J.J., et al. 2023. Emergence of Consciousness from Anesthesia Through Ubiquitin Degradation of KCC2 in the Ventral Posteromedial Nucleus of the Thalamus. Nature Neuroscience, . |
| 36889319 | 1:750 | Jarvis, R., et al. 2023. Direct Activation of KCC2 Arrests Benzodiazepine Refractory Status Epilepticus and Limits the Subsequent Neuronal Injury in Mice. Cell Reports Medicine, 100957. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 29092909 | 1:1000 | Conway, L.C., et al. 2017. N-Ethylmaleimide increases KCC2 cotransporter activity by modulating transporter phosphorylation. Journal of Biological Chemistry, 292(52), pp.21253-21263. |
Product Specific Protocols
- Western Blot Protocol: Download