Our Anti-Polo-Like Kinase Kinase (Ser482,486,490) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat and Xenopus Polo-Like Kinase Kinase (Ser482,486,490) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units overnight).
Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser-482, Ser-486 and Ser-490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser-482,486,490 in PLKK.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
PLKK1
120 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 of Xenopus polo-like kinase kinase (PLKK), conjugated to keyhole limpet hemocyanin (KLH).
Xenopus
Human, Mouse, Zebrafish
AB_2492210
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~120k PLKK protein phosphorylated at Ser482,486,490.
Phosphorylated
Ser482,486,490
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Polo like kinase kinase 1 antibody, Serine/threonine kinase 10 antibody, stk10 a antibody, xPlkk1 antibody
Erikson, E., Haystead, T.A., Qian, Y.W. and Maller, J.L., 2004. A feedback loop in the polo-like kinase activation pathway. Journal of Biological Chemistry, 279(31), pp.32219-32224. PMID: 15166215
Kumagai, A. and Dunphy, W.G., 1996. Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts. Science, 273(5280), pp.1377-1380. PMID: 8703070
Liu, J., Lewellyn, A.L., Chen, L.G. and Maller, J.L., 2004. The polo box is required for multiple functions of Plx1 in mitosis. Journal of Biological Chemistry, 279(20), pp.21367-21373. PMID: 15016807
van Vugt, M.A., van de Weerdt, B.C., Vader, G., Janssen, H., Calafat, J., Klompmaker, R., Wolthuis, R.M. and Medema, R.H., 2004. Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis. Journal of biological chemistry, 279(35), pp.36841-36854. PMID: 15210710
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