Anti-PKCα (Central region) Antibody (M237)

Name: Anti-PKCα (Central region) Antibody
Brand: PhosphoSolutions
SKU: PM2371
Price: 360.0 USD
Availability: InStock

Our PKCα (Central region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat PKCα (Central region) and is Protein A purified. It is great for use in WB, ICC, IHC, IP.

Formalin fixed, citric acid treated parafin sections of adult mouse brain. Sections were probed with anti-PKCα (PM2371) then anti-mouse:HRP before detection using DAB. (Image provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKCα antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKCα (Central region) or anti-phospho-PKCα (Ser-657/Tyr-658). The latter shows no detection of PKCα after phosphatase treatment.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

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SKU: PM2371

Volume: 100 µL

Variant

Price:

$360.00

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Product Details

Target

PKCα (Central region)

Target Description

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCα and PKCβI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M237

Isotype

IgG2b

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

PRKCA

Molecular Weight

Antigen

Clone (M237) was generated from a recombinant human PKCα that included amino acids residues in the central region. This region is highly conserved in rat and mouse PKCα, and has homology to conserved regions in PKCβ.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 6:00
IP: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects an 82kDa* protein corresponding to the molecular mass of PKCα on SDS-PAGE immunoblots of neonatal rat brain and adult mouse brain lysates.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PKC alpha

UniProt Details

UniProt (Human): P17252
UniProt (Immunogen Species): P17252

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Anti-PKCα (Central region) Antibody (M237)

SKU: PM2371

Product Details

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