Anti-PI3 Kinase, p85 (C-terminal region) Antibody (M253)

Name: Anti-PI3 Kinase, p85 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: PM2531
Price: 379.0 USD
Availability: InStock

Our PI3 Kinase, p85 (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat PI3 Kinase, p85 (C-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI3 Kinase, p85 (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).

Immunocytochemical labeling of PI3 Kinase p85 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal PI3 Kinase p85 (C-terminal region) antibody (PM2531), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI3 Kinase, p85 (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).

Click on image to zoom

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Mouse

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Data Sheet

SKU: PM2531

Volume: 100 µL

Variant

Price:

$379.00

Quantity:

Ships: 1-2 business days

Product Details

Target

PI3 Kinase, p85 (C-terminal region)

Scientific Background

Phosphoinositide 3-kinase (PI3K) phosphorylates phosphatidylinositol (PI), PI-4-phosphate and PI-4,5-bisphosphate to catalyze the production of PI-3,4,5-triphosphate. Growth factors and hormones activate PI3K to coordinate various cellular events, such as cell growth, cell cycle entry, cell migration and cell survival. This PI3K activation is reversed by PTEN. In cancers caused by the loss of PTEN activity, PI3K is constituitively active and promotes cell proliferation. PI3Ks are composed of a catalytic subunit and a regulatory subunit. Various isoforms of the catalytic subunit (p110a, p110b and p110d) associate with regulatory subunits (p85a and p85b) with the exception of p110g which interacts with a unique p101 regulatory subunit.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M253

Isotype

IgG2a

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

PIK3R1

Molecular Weight

Immunogen

Clone M253 was generated from a recombinant protein corresponding to amino acids in the C-terminal region of human PI3 Kinase α subunit. This region is homologous to similar regions in rat and mouse PI3 Kinase α subunit.

Immunogen Species

Human

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects an 85 kDa* band corresponding to the p85 subunit of PI3 Kinase in western blots of human A431 and Jurkat cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

AGM7; PI3K; IMD36; p85; p85-ALPHA; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha

UniProt ID

P27986
UniProt Summary: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.

NCBI Gene ID

5295

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Anti-PI3 Kinase, p85 (C-terminal region) Antibody (M253)

SKU: PM2531

Product Details

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