Anti-Phosphotyrosine Antibody

Name: Anti-Phosphotyrosine Antibody
Brand: PhosphoSolutions
SKU: PP2221
Price: 275.0 USD
Availability: InStock

2 Citations

Our Anti-Phosphotyrosine rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Anti-Phosphotyrosine and is antigen affinity purified. It is great for use in WB, ICC, IP.

Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Bar graph showing rabbit polyclonal anti-Phosphotyrosine (PP2221) binding to a variety of phosphotyrosine containing peptides, but no binding to unphosphorylated peptide (beta-Catenin (a.a. 29-45).

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Rabbit

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SKU: PP2221

Volume: 100 µL

Variant

Price:

$275.00

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Product Details

Target

Anti-Phosphotyrosine

Target Description

Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IP, WB

Host Species

Rabbit

Gene Name

N/A

Molecular Weight

N/A

Antigen

Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved phosphotyrosine motifs.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

p-Tyr

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Phosphotyrosine mAb

UniProt Details

UniProt (Immunogen Species): N/A

Product Specific References for Applications and Species

Western Blot: Mouse | Rabbit

Western Blot: Mouse
PMID	Dilution	Publication
33116123	1:1000	Kim, J, et al. 2020. Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nature communications, 5436.

Western Blot: Rabbit
PMID	Dilution	Publication
22427066	1:1000	Phoungpetchara, I., et al. 2012. Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii. Cell Tissue Research, 609-623.

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Anti-Phosphotyrosine Antibody

SKU: PP2221

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Phosphotyrosine Antibody

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