Anti-Phosphotyrosine Antibody
Our Anti-Phosphotyrosine rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Anti-Phosphotyrosine and is antigen affinity purified. It is great for use in WB, ICC, IP.
SKU: PP2221
Product Details
Anti-Phosphotyrosine
Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.
Antigen Affinity Purified
Polyclonal
IgG
ICC, IP, WB
Rabbit
N/A
N/A
Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved phosphotyrosine motifs.
Chicken, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:100
IP: 1:100
ICC: 1:100
IP: 1:100
Unconjugated
This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.
Phosphorylated
p-Tyr
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Phosphotyrosine mAb
UniProt (Immunogen Species): N/A
Product Specific References for Applications and Species
Western Blot: Mouse | ||
PMID | Dilution | Publication |
33116123 | 1:1000 | Kim, J, et al. 2020. Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nature communications, 5436. |
Western Blot: Rabbit | ||
PMID | Dilution | Publication |
22427066 | 1:1000 | Phoungpetchara, I., et al. 2012. Expression of the male reproduction-related gene (Mar-Mrr) in the spermatic duct of the giant freshwater prawn, Macrobrachium rosenbergii. Cell Tissue Research, 609-623. |