Anti-Phosphotyrosine Antibody (M375)

Name: Anti-Phosphotyrosine Antibody
Brand: PhosphoSolutions
SKU: PM3751
Price: 275.0 USD
Availability: InStock

1 Citation

Our Anti-Phosphotyrosine phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Anti-Phosphotyrosine and is Protein A purified. It is great for use in WB, ICC, IP.

Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot of HeLa cells treated with pervanadate (1 mM) for 30 min. Phosphotyrosine containing proteins were immunoprecipitated with rabbit polyclonal anti-Phosphotyrosine:Agarose (Lane 1) or with no antibody agarose beads (Lane 2), and blots were made that included the whole lysate (Lane 3). The blots were probed with mouse monoclonal anti-Phosphotyrosine (PM3751) to detect phosphotyrosine containing proteins.

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Mouse

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SKU: PM3751

Volume: 100 µL

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Price:

$275.00

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Product Details

Target

Anti-Phosphotyrosine

Target Description

Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M375

Isotype

IgG2b

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

N/A

Molecular Weight

N/A

Antigen

Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.

Post Translational Modification Type

Phosphorylated

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Phosphotyrosine mAb

UniProt Details

UniProt (Immunogen Species): N/A

Product Specific References for Applications and Species

Immunocytochemistry: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
34637702	not listed	Liu, B, et al. 2021. Biosensors based on peptide exposure show single molecule conformations in live cells. Cell, 5670-5685.

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Anti-Phosphotyrosine Antibody (M375)

SKU: PM3751

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Phosphotyrosine Antibody

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