Anti-Peroxiredoxin-6 (NSGPx) Antibody

Name: Anti-Peroxiredoxin-6 (NSGPx) Antibody
Brand: Biosensis
SKU: R-169-100
Price: 347.0 USD
Availability: InStock

Our Anti-Peroxiredoxin-6 (NSGPx) rabbit polyclonal primary antibody detects human, mouse, and rat Peroxiredoxin-6 (NSGPx), and is whole serum. It is validated for use in ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-169-100

Volume/Size: 100 µL

Variant

Price:

$347.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Peroxiredoxin-6 (NSGPx)

Scientific Background

FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Immunogen

Rat recombinant Peroxiredoxin-6. The sequence is homologous in human and mouse peroxiredoxin-6.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.

Physical State

Lyophilized

Dilution Ranges

WB: 1:100-1:1000
IHC: 1:1000-1:4000
ELISA: 1:1000-1:4000

Application Details

This antibody works in IHC on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. This antiserum stains the cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. It stains human brain astrocytes in Parkinson's and Alzheimer 's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies. Other tissues have not yet been tested.

Conjugate

Unconjugated

Specificity

This antibody has been shown to be specific for Peroxiredoxin-6 protein. Rat, human and mouse, other species have not yet been tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

peroxiredoxin-6; Non-selenium glutathione peroxidase; 1-Cys peroxiredoxin; antioxidant protein 2; NSGPx; acidic calcium-independent phospholipase A2; 1-Cys PRX; aiPLA2; Thiol-specific antioxidant protein; Prdx6; Aop2; Tsa

UniProt ID

O35244
UniProt Summary: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Also has phospholipase activity, can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH. Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis. Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate.

NCBI Gene ID

94167

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Anti-Peroxiredoxin-6 (NSGPx) Antibody

SKU: R-169-100

Product Details

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