Our Anti-Peripherin primary antibody from PhosphoSolutions is mouse monoclonal. It detects mouse and rat Peripherin and is concentrated tissue culture supernatant. It is great for use in WB, IHC, ICC.
Immunostaining of cultured newborn rat neurons and glia showing peripherin (cat. 1630-PER, green, 1:200) and neurofilament L (red). The nuclear DNA is stained blue.
Peripherin is a ~57 kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. In contrast to the neurofilaments, peripherin is strongly up-regulated after nerve injury (1). Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors (2). Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes (3). Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.
Concentrated tissue culture supernatant
Monoclonal
7C5
IgG1
ICC, IHC, WB
Mouse
PRPH
57 kDa
Recombinant rat peripherin expressed in and purified from E. coli.
Mouse, Rat
AB_2492207
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Concentrated tissue culture supernatant
Concentrated tissue culture supernatant + 10 mM NaN3
Specific for endogenous levels of the ~57 kDa peripherin protein. This antibody performs well on aldehyde fixed tissues.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Terao E, Janssens S, van den Bosch de Aquilar P, Portier M, Klosen P (2000) In vivo expression of the intermediate filament peripherin in rat motorneurons: modulation by inhibitory and stimulatory signals. Neuroscience 101(3):679-88. PMID: 11113316
Roberson J, Doroudchi MM, Nguyen MD, Durham, HD, Shaw G, Julien JP, Mushynski WE. (2003) A neurotoxic peripherin splice variant in a mouse model of ALS. Journal of Cell Biology 160(6):939-49. PMID: 12642616
Boitard C, Villa MC, Becourt C, Gia HP, Huc C, Sempe P, Portier MM, Bach JF (1992) Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products. Proc Natl Acad Sci USA 89(1):172-6. PMID: 1729686
Related Products
Recently Viewed
Your research is our top priority
Highly-validated antibodies and reagents from our family of brands.
