Full-length recombinant peripherin was injected into laying hens, and then the IgY fraction was purified from hyperimmunized animals. These antibodies have been validated with human, mouse and rat tissues.
Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).
Human Perpherin is a 53,651 dalton protein (470 amino acids) expressed in all PNS neurons and some CNS neurons, including a subset of cortical neurons, hippocampal neurons, and ventral horn spinal cord motor neurons. At an ultrastructural level, peripherin immunoreactivity is associated with the neurofilament portion of the cytoskeleton, being particularly abundant in the cell body. Peripherin protein has been shown to undergo upregulation during periods of trophic stress in cultured neurons, and mutations of the peripherin gene have been associated with the neurodegenerative disease amyotrophic lateral sclerosis.
IgY Fraction
2 mg/mL
Polyclonal
IgY
ICC, IHC, WB
Chicken
PRPH NEF4 PRPH1
60 kDa
Recombinant rat peripherin expressed in Escherichia coli
Human, Mouse, Rat
AB_2313558
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Liquid
Chickens were immunized with full-length recombinant peripherin protein. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:10000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Alsaloum, M., et al. 2021. Contributions of NaV1.8 and NaV1.9 to excitability in human induced pluripotent stem-cell derived somatosensory neurons. Scientific Reports, 24283.
Rose, K.E., et al. 2013. Immunohistological demonstration of CaV3. 2 T-type voltage-gated calcium channel expression in soma of dorsal root ganglion neurons and peripheral axons of rat …. Neuroscience, 263-274.
Akin, E.J., et al. 2019. Building sensory axons: Delivery and distribution of NaV1.7 channels and effects of inflammatory mediators. Science Advances, eaax4755.
Chamessian, A.G., et al. 2017. 5-Hydroxymethylcytosine (5hmC) and Ten-eleven translocation 1–3 (TET1–3) proteins in the dorsal root ganglia of mouse: Expression and dynamic regulation in neuropathic pain. Somatosensory & Motor Research, 72-79.
Baxter, J.C., et al. 2014. Functional expression of α7-nicotinic acetylcholine receptors by muscle afferent neurons. Journal of Neurophysiology, 1549-1558.
Persson, A.K., et al. 2011. Nav1. 7 accumulates and co-localizes with phosphorylated ERK1/2 within transected axons in early experimental neuromas. Experimental Neurology, 273-279.
