Name: Anti-Peripherin Antibody
Brand: Aves Labs
SKU: PER
Price: 265.0 USD
Availability: InStock

Full-length recombinant peripherin was injected into laying hens, and then the IgY fraction was purified from hyperimmunized animals. These antibodies have been validated with human, mouse and rat tissues.

Clonality

Polyclonal

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

PRPH NEF4 PRPH1

Molecular Weight

60 kDa

Go to Full Product Details

Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).

Product Details

Target

Perpherin

Target Description

Human Perpherin is a 53,651 dalton protein (470 amino acids) expressed in all PNS neurons and some CNS neurons, including a subset of cortical neurons, hippocampal neurons, and ventral horn spinal cord motor neurons. At an ultrastructural level, peripherin immunoreactivity is associated with the neurofilament portion of the cytoskeleton, being particularly abundant in the cell body. Peripherin protein has been shown to undergo upregulation during periods of trophic stress in cultured neurons, and mutations of the peripherin gene have been associated with the neurodegenerative disease amyotrophic lateral sclerosis.

Concentration

2 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

PRPH NEF4 PRPH1

Molecular Weight

60 kDa

Antigen

Recombinant rat peripherin expressed in Escherichia coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313558

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with full-length recombinant peripherin protein. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.

Dilution Ranges

WB: 1:2000-1:5000
IHC: 1:2000-1:5000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:10000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

Ice packs

Synonyms

Peripherin (Neurofilament 4)

UniProt Details

UniProt (Immunogen Species): P41219

Citations (4)

Baxter JC, Ramachandra R, Mayne DR, Elmslie KS. (2014), 'Functional expression of α7-nicotinic acetylcholine receptors by muscle afferent neurons.' Journal of Neurophysiology. 10.1152/jn.00035.2014.
Ramachandra R, Elmslie KS. (2016), 'Voltage-dependent sodium (NaV) channels in group IV sensory afferents .' Molecular Pain. 10.1177/1744806916660721.
Olson W, Abdus-Saboor I#, Cui L#, Burdge J, Raabe T, Ma M, Luo W. (2017), 'Sparse genetic tracing reveals regionally specific functional organization of mammalian nociceptors.' eLife. 10.7554/eLife.29507.001.
Chamessian AG, Qadri YJ, Cummins M, Hendrickson M, Berta T, Buchheit T, Van de Ven T. (2017), '5-Hydroxymethylcytosine (5hmC) and Ten-eleven translocation 1–3 (TET1–3) proteins in the dorsal root ganglia of mouse: Expression and dynamic regulation in neuropathic pain.' Somatosensory & Motor Research. 10.1080/08990220.2017.1292237.

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Anti-Peripherin Antibody

Full-length recombinant peripherin was injected into laying hens, and then the IgY fraction was purified from hyperimmunized animals. These antibodies have been validated with human, mouse and rat tissues.