Our Anti-Periostin pan primary antibody from PhosphoSolutions is rabbit polyclonal. It detects chicken, human, mouse, and rat Periostin pan and is antigen affinity purified. It is great for use in WB, IHC.
Western blot of rat lung lysate showing specific immunolabeling of the ~93 kDa periostin protein triplet.
Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has recently been shown to promote collagen fibrogenesis, inhibit differentiation of progenitor cells into cardiomyocytes and to be essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008)
Antigen Affinity Purified
Polyclonal
IgG
IHC, WB
Rabbit
POSTN
93 kDa
Synthetic peptide corresponding to amino acid residues from the fasciclin domain 1 of mouse periostin.
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
PBS
WB: 1:1000
IHC: 1:100
Unconjugated
Specific for endogenous levels of the three periostin bands around ~93 kDa. The immunogenic peptide is present in all known splice variants of periostin and therefore this antibody recognizes all known molecular forms of periostin. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH6.0)/ 0.1 % Tween-20).
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Kruzynska‐Frejtag, A., Wang, J., Maeda, M., Rogers, R., Krug, E., Hoffman, S., Markwald, R.R. and Conway, S.J., 2004. Periostin is expressed within the developing teeth at the sites of epithelial–mesenchymal interaction. Developmental dynamics: an official publication of the American Association of Anatomists, 229(4), pp.857-868. PMID: 15042709
Norris, R.A., Borg, T.K., Butcher, J.T., Baudino, T.A., Banerjee, I. and Markwald, R.R., 2008. Neonatal and adult cardiovascular pathophysiological remodeling and repair: developmental role of periostin. Annals of the New York Academy of Sciences, 1123(1), pp.30-40. PMID: 18375575
Norris, R.A., Damon, B., Mironov, V., Kasyanov, V., Ramamurthi, A., Moreno‐Rodriguez, R., Trusk, T., Potts, J.D., Goodwin, R.L., Davis, J. and Hoffman, S., 2007. Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. Journal of cellular biochemistry, 101(3), pp.695-711. PMID: 17226767
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