Anti-Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) Antibody

Name: Anti-Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) Antibody
Brand: Biosensis
SKU: C-1398-50
Price: 337.0 USD
Availability: InStock

Our Anti-Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) chicken polyclonal primary antibody detects cat, human, mouse, other mammals (predicted), and rat Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1), and is IgY preparation. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.

HeLa cells stained with Chicken polyclonal antibody to Peptidylprolyl isomerase (1:1,000 dilution, green) and Mouse monoclonal antibody to Fibrillarin (Nop1p) M-1372-250 (red). Peptidylprolyl isomerase (Pin-1) stains the nuclear matrix and, much more faintly, the cytoplasm. The fibrillarin antibody marks nucleoli.

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Species Reactivity

Feline, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1398-50

Volume/Size: 50 µL

Variant

Price:

$337.00

Quantity:

Ships: 5-7 business days

Product Details

Target

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1)

Scientific Background

The enzyme Peptidylprolyl isomerase (Pin1) is responsible for flipping the proline ring from the cis to trans conformation. This enzyme regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity (ref: SWISSPROT). Pin1 is concentrated in the nucleus in small punctate structures and is particularly obvious in tumor cells.

Format

IgY preparation

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Molecular Weight

21 kDa

Immunogen

Recombinant full length Peptidylprolyl isomerase (Pin1) purified from E.coli

Immunogen Species

Human

Species Reactivity

Feline, Human, Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized IgY preparation, with sodium azide.

Dilution Ranges

WB: 1:5000-1:10000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB. This antibody detects ~21 kDa Pin1 protein. Human, rat, mouse and feine. Predicted to react with other mammalian tissue.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

DOD; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; PPIase Pin1; Peptidyl-prolyl cis-trans isomerase Pin1; rotamase Pin1; PPIase Pin-1; PIN1

UniProt ID

Q13526
UniProt Summary: Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes. Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner. Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN. May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR). Upon IL33-induced lung inflammation, catalyzes cis-trans isomerization of phosphorylated IRAK3/IRAK-M, inducing IRAK3 stabilization, nuclear translocation and expression of pro-inflammatory genes in dendritic cells. Catalyzes cis-trans isomerization of phosphorylated phosphoglycerate kinase PGK1 under hypoxic conditions to promote its binding to the TOM complex and targeting to the mitochondrion. Acts as a negative regulator of adipocyte browning by binding to phosphorylated PRDM16, targeting PRDM16 for degradation.

NCBI Gene ID

5300

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25°C (ambient)

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Anti-Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (Pin1) Antibody

SKU: C-1398-50

Product Details

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