Anti-PD-L1 (Extracellular region) M080 Antibody (M080)

Name: Anti-PD-L1 (Extracellular region) M080 Antibody
Brand: PhosphoSolutions
SKU: PM0801
Price: 379.0 USD
Availability: InStock

Our PD-L1 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human PD-L1 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.

Western blot of human recombinant PD-L1 extracellular region (40 kDa; lanes 1 & 3) and endogenous PD-L1 in human lung (lanes 2 & 4). The blot was probed with mouse monoclonals anti-PD-L1 (PM0511) at 1:500 (lanes 1 & 2) and anti-PD-L1 (PM0801) at 1:250 (lanes 3 & 4).

Immunocytochemical labeling of PD-L1 in aldehyde fixed human MDA-MB-231 carcinoma cells untreated (A) or treated with 100 ng/ml IFNγ for 48 hrs (B). The cells were labeled with mouse monoclonal anti-PD-L1 (PM0801). The antibody was detected using goat anti-mouse DyLight® 594.

Representative Standard Curve using mouse monoclonal anti-PD-L1 (PM0801) for ELISA capture of human recombinant PD-L1 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.

Western blot of human recombinant PD-L1 extracellular region (40 kDa; lanes 1 & 3) and endogenous PD-L1 in human lung (lanes 2 & 4). The blot was probed with mouse monoclonals anti-PD-L1 (PM0511) at 1:500 (lanes 1 & 2) and anti-PD-L1 (PM0801) at 1:250 (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

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Data Sheet

SKU: PM0801

Volume: 100 µL

Variant

Price:

$379.00

Quantity:

Ships: 1-2 business days

Product Details

Target

PD-L1 (Extracellular region)

Scientific Background

Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The B7 family members have conserved regions that include extracellular IgV and IgC domains, and a short cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas. PD-L1 plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, which promotes tumor survival. The blockage of the PD1/PD-L1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. Several therapies that target PD1/PD-L1 pathways are currently in use or in clinical trials

Format

Protein G Purified

Clonality

Monoclonal

Clone

M080

Isotype

IgG1

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

Gene Name

CD274

Molecular Weight

45-55

Immunogen

Clone (M080) was generated from a recombinant protein that included the extracellular region of human PD-L1 protein.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:200
IP: 1:200

Conjugate

Unconjugated

Specificity

Clone M080 mouse monoclonal antibody detects a 45-55 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human recombinant PD-L1 extracellular region, and human lung tissue. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

B7-H; Programmed cell death 1 ligand 1; PD-L1; PDCD1L1; PDCD1LG1; PDL1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; Programmed death ligand 1; hPD-L1; B7-H1; CD274; B7H1

UniProt ID

Q9NZQ7
UniProt Summary: Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis.

NCBI Gene ID

29126

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Anti-PD-L1 (Extracellular region) M080 Antibody (M080)

SKU: PM0801

Product Details

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