Anti-Paxillin (Thr-538), Phosphospecific Antibody

Name: Anti-Paxillin (Thr-538), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: PP4491
Price: 329.0 USD
Availability: InStock

1 Citation

Our Paxillin (Thr-538) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Paxillin (Thr-538) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of human A431 cells treated with Calyculin A (100 nm) for 30 min (lanes 1-6). The blot was treated with lambda phosphatase (lanes 2, 4, & 6), then probed with rabbit polyclonal anti-Paxillin (C-terminal; PP1161) (lanes 1 & 2), anti-phospho-Paxillin (Thr-538; PP4491) (lanes 3 & 4), and mouse monoclonal anti-Paxillin (PM1071) (lanes 5 & 6).

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: PP4491

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

Paxillin (Thr-538)

Target Description

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at tyrosine, threonine, and serine sites. Serine and threonine phosphorylation of paxillin occur in response to growth-factor activation, PKC activators, and fibronectins. Phosphorylation of Ser-85, Ser-178, and Thr-538 may be important sites for regulating paxillin activity. Paxillin phosphorylation of Thr-538 occurs in response to TPA-activated PKCs in vitro, and this phosphorylation may contribute to dissolution of the actin cytoskeleton and redistribution of LFA-1 integrins in vivo.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

PXN

Molecular Weight

Antigen

Phospho-Paxillin (Thr-538) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 538 from human paxillin a. This sequence is highly conserved in rat and mouse paxillin and is also found in the other paxillin isoforms (b & g).

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr-538

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

UniProt Details

UniProt (Human): P49023
UniProt (Immunogen Species): P49023

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
32939232	not listed	Bello-Gamboa, A, et al. 2020. Actin reorganization at the centrosomal area and the immune synapse regulates polarized secretory traffic of multivesicular bodies in T lymphocytes. Journal of extracellular vesicles, 1759926.

Western Blot: Human
PMID	Dilution	Publication
32939232	not listed	Bello-Gamboa, A, et al. 2020. Actin reorganization at the centrosomal area and the immune synapse regulates polarized secretory traffic of multivesicular bodies in T lymphocytes. Journal of extracellular vesicles, 1759926.

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Anti-Paxillin (Thr-538), Phosphospecific Antibody

SKU: PP4491

Product Details

Product Specific References for Applications and Species

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