Anti-Parvalbumin Recombinant Chicken Chimeric mAb (L114/81)
Our chicken Anti-Parvalbumin recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L114/81. It detects mouse and rat Parvalbumin and is purified by affinity chromatography. Works in AT, ELISA, ICC, IHC, WB.
Representative image of 4% paraformaldehyde fixed free-floating rat hippocampus sections stained with Aves Labs anti-Parvalbumin recombinant chicken monoclonal antibody (red, Cat No. 78-479) at 1:1000 dilution and co-stained with anti-Iba1 rabbit polyclonal antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-Parvalbumin specifically stains the GABAnergic interneurons in the cerebellum. Image courtesy of EnCor Biotechnology.
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Mouse, Rat
AT, ELISA, ICC, IHC, WB
Chicken
SKU: 78-479
Ships: 1-2 business days
Product Details
Parvalbumin
Parvalbumin (PA) is a classical small, mostly cytosolic Ca2+-binding protein of the EF-hand superfamily expressed in vertebrates in a tissue- and cell-specific manner, serving as a magnesium/ calcium buffer (Permyakov EA et al., 2017). Parvalbumin (PV)-expressing interneurons are a class of inhibitory neurons that fire rapid barrages of action potentials, and are accordingly named “fast-spiking” interneurons (Trachtenberg J. 2015).
Purified by affinity chromatography
1 mg/mL
Recombinant
L114/81
IgY
AT, ELISA, ICC, IHC, WB
Chicken
Pvalb Pva
12 kDa
Fusion protein amino acids 1-110 (full-length) of rat Parvalbumin (accession number P02625) produced recombinantly in E. Coli
Rat
Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of our NeuroMab clone L114/81 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L114/81 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by Western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
bA122O1.1; Parvalbumin alpha; GABA and glycine transporter; hVIAAT; SLC32A1; SLC32A 1; solute carrier family 32 (GABA vesicular transporter member 1; Solute carrier family 32 member 1; Vesicular GABA Amino Acid Transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; VGAT; VIAAT; VIAAT_HUMAN
Q9H598
UniProt Summary: Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals. The transport is equally dependent on the chemical and electrical components of the proton gradient. May also transport beta-alanine. Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake.
UniProt Summary: Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals. The transport is equally dependent on the chemical and electrical components of the proton gradient. May also transport beta-alanine. Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake.
140679
Shipped on ice packs

