Anti-Parkinson disease protein 2 (Parkin) Antibody

Name: Anti-Parkinson disease protein 2 (Parkin) Antibody
Brand: Biosensis
SKU: R-113-100
Price: 327.0 USD
Availability: InStock

Our Anti-Parkinson disease protein 2 (Parkin) rabbit polyclonal primary antibody detects guinea pig, human, and rat Parkinson disease protein 2 (Parkin), and is whole serum. It is validated for use in ELISA, IHC-Frozen, WB.

Immunohistochemical staining of parkin in rat brainstem using rabbit polyclonal antibody to human parkin, catalogue number R-113-100. Trigeminal motor neurons are intensely stained.

Parkin immunoreactivity: Lane 1, rat brain extract immunoblotted with rabbit polyclonal antibody to human parkin, catalogue number R-113-100; Lane 2, rat brain extract preincubated with the parkin immunogen peptide and immunoblotted with R-113-100.

Immunohistochemical staining of parkin in rat brainstem using rabbit polyclonal antibody to human parkin, catalogue number R-113-100. Trigeminal motor neurons are intensely stained.

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Species Reactivity

Guinea Pig, Human, Pig, Rat

Applications

ELISA, IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-113-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Parkinson disease protein 2 (Parkin)

Scientific Background

FUNCTION: Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP and SEPT5. May play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Regulates cyclin E during neuronal apoptosis. May represent a tumor suppressor gene. SUBCELLULAR LOCATION: Cytoplasm. Co-localizes with STY11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. TISSUE SPECIFICITY: Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ELISA, IHC, WB

Host Species

Rabbit

Molecular Weight

50 kDa

Immunogen

A synthetic peptide (RILGEEQYNRYQQYGAEEC) as part of human Parkin conjugated to diphtheria toxoid has been used as the immunogen.

Immunogen Species

Human

Species Reactivity

Guinea Pig, Human, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Dilution Ranges

WB: 1:500-1:2000
IHC: 1:500-1:2000
ICC: 1:500-1:2000

Application Details

This antiserum stains trigeminal motor neurons in rat brain stem.

Conjugate

Unconjugated

Specificity

This antiserum is known to specifically recognize Parkin shown by IHC and WB. This antibody is known to react with Parkin of guinea pig and rat.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Ubiquitin E3 ligase PRKN; Parkinson juvenile disease protein 2; Parkinson disease protein 2; PARK2; PRKN

UniProt ID

O60260

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25°C (ambient)

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Anti-Parkinson disease protein 2 (Parkin) Antibody

SKU: R-113-100

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