Our Anti-PAK-1 (Thr84) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat PAK-1 (Thr84) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68 kDa PAK1 protein phosphorylated at Thr84 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase , 800 units/1mg protein for 30 minutes).
PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation (Ye et al, 2012). Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif (Chen et al, 2004). OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl (Chen et al, 2004). It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. (Chen et al, 2004). It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at Thr-84 and bind to gelsolin (Chen et al, 2004).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
PAK1
68 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 of human PAK1, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~68 kDa PAK1 phosphorylated at Thr84. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Thr84
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Chen, W., Yazicioglu, M. and Cobb, M.H., 2004. Characterization of OSR1, a member of the mammalian Ste20p/germinal center kinase subfamily. Journal of Biological Chemistry, 279(12), pp.11129-11136. PMID: 14707132
Ye, D.Z. and Field, J., 2012. PAK signaling in cancer. Cellular Logistics, 2(2), pp.105-116. PMID: 23162742
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