Anti-p38α MAP Kinase (C-terminal) M138 Antibody (M138)

Name: Anti-p38α MAP Kinase (C-terminal) M138 Antibody
Brand: PhosphoSolutions
SKU: PM1381
Price: 360.0 USD
Availability: InStock

1 Citation

Our p38α MAP Kinase (C-terminal) M138 mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat p38α MAP Kinase (C-terminal) M138 and is Protein A purified. It is great for use in WB, ICC.

Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38α (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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SKU: PM1381

Volume: 100 µL

Variant

Price:

$360.00

Quantity:

Product Details

Target

p38α MAP Kinase (C-terminal) M138

Target Description

p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (α,β,γ,δ) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. T cells possess an alternative pathway for p38 activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M138

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

Mapk14

Molecular Weight

Antigen

Clone M138 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of rat p38α. This sequence is identical in human and mouse p38α.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

This antibody detects a 38 kDa* protein corresponding to the apparent molecular mass of p38α on SDS-PAGE immunoblots of human Jurkat, A431, and HeLa cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

MAPK, p38, p38alpha, p38MAPK

UniProt Details

UniProt (Human): Q16539
UniProt (Immunogen Species): P70618

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
31155581	not listed	Nakahara, K, et al. 2019. Attenuation of Macrophage Migration Inhibitory Factor-Stimulated Signaling via S-Nitrosylation. Biological & pharmaceutical bulletin, 1044-1047.

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Anti-p38α MAP Kinase (C-terminal) M138 Antibody (M138)

SKU: PM1381

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-p38α MAP Kinase (C-terminal) M138 Antibody

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