Anti-p21/CIP1/WAF1 Antibody (M513)

Name: Anti-p21/CIP1/WAF1 Antibody
Brand: PhosphoSolutions
SKU: CM5131
Price: 292.0 USD
Availability: InStock

Our p21/CIP1/WAF1 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human p21/CIP1/WAF1 and is antigen affinity purified. It is great for use in WB, ICC, IHC, IP.

Western blot analysis of p21 expression in human endothelial cells (lanes 1 & 2). The blots were probed with mouse monoclonal anti-p21 at 1:250 (lane 1) and 1:1000 (lane 2).

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Species Reactivity

Human

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

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Data Sheet

SKU: CM5131

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

p21/CIP1/WAF1

Scientific Background

The tumor suppressor protein p21/CIP1/WAF1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression. Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element. Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation.

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M513

Isotype

IgG2a

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

CDKN1A

Molecular Weight

Immunogen

Clone M513 was generated from a full length recombinant human p21 protein. The antibody detects human p21.

Immunogen Species

Human

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
IHC: 1:50
ICC: 1:50
IP: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of p21 on SDS-PAGE immunoblots of human endothelial cells (HUVEC), HeLa cells, and Fibroblasts. The antibody is also useful for immunoprecipitation and immunofluorescent labeling in human cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

CAP20; Cyclin-dependent kinase inhibitor 1; CIP1; MDA-6; P21; SDI1; WAF1; p21CIP1; CDK-interacting protein 1; CDK-interaction protein 1; DNA synthesis inhibitor; cyclin-dependent kinase inhibitor 1A (p21; melanoma differentiation associated protein 6; wild-type p53-activated fragment 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6 p21 CDKN1A CAP20; CDKN1; MDA6; PIC1

UniProt ID

P38936
UniProt Summary: Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Negatively regulates the CDK4- and CDK6-driven phosphorylation of RB1 in keratinocytes, thereby resulting in the release of E2F1 and subsequent transcription of E2F1-driven G1/S phase promoting genes.

NCBI Gene ID

1026

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Anti-p21/CIP1/WAF1 Antibody (M513)

SKU: CM5131

Product Details

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