Our Anti-Olig2 primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat Olig2 and is protein a purified. It is great for use in WB, IHC, ICC, IP.
Immunostaining of primary rat cortical neuroepithelial cells with anti-Olig2 (cat. 1538-OLIG2, red, 1:1000). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).
Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007)
Protein A purified
Polyclonal
IgG
ICC, IHC, IP, WB
Rabbit
OLIG2
27 kDa
Recombinant mouse Olig2.
Human, Mouse, Rat
AB_2492193
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Specific for endogenous levels of the ~32 kDa Olig2 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Basic domain helix loop helix protein class B 1 antibody, Basic helix loop helix protein class B 1 antibody, BHLHB antibody, bHLHB1 antibody, bHLHe19 antibody, Class B basic helix loop helix protein 1 antibody, Class B basic helix-loop-helix protein 1 antibody, class E basic helix loop helix protein 19 antibody, Class E basic helix-loop-helix protein 19 antibody, Human protein kinase C binding protein RACK17 antibody, Olig2 antibody, OLIG2_HUMAN antibody, Oligo2 antibody, Oligodendrocyte lineage transcription factor 2 antibody, Oligodendrocyte specific bHLH transcription factor 2 antibody, Oligodendrocyte transcription factor 2 antibody, OTTHUMP00000067569 antibody, OTTHUMP00000067570 antibody, PRKCBP2 antibody, Protein kinase C binding protein 2 antibody, Protein kinase C binding protein RACK17 antibody, Protein kinase C-binding protein 2 antibody, Protein kinase C-binding protein RACK17 antibody, RACK17 antibody
Xu, R., et al. 2019. OLIG2 drives abnormal neurodevelopmental phenotypes in human iPSC-based organoid and chimeric mouse models of down syndrome. Cell Stem Cell, 24(6), pp.908-926.
Kim, H., et al. 2019. Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin. STAR protocols, 2(2), p.100443.
Kim, H., et al. 2019. Pluripotent Stem Cell-derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins. Stem cell reports, 12(5), pp.890-905.
LeBlanc, V.G., et al. 2022. Single-cell landscapes of primary glioblastomas and matched explants and cell lines show variable retention of inter- and intratumor heterogeneity. Cancer Cell, 40(4), pp.379-392.
Mauney, S.A., et al. 2015. Differentiation of oligodendrocyte precursors is impaired in the prefrontal cortex in schizophrenia. Schizophrenia Research, 169(1), 374-380.
Kucharova, K., et al. 2017. Distinct NG2 proteoglycan-dependent roles of resident microglia and bone marrow-derived macrophages during myelin damage and repair. PloS one, 12(11), p.e0187530.
Kucharova, K., et al. 2015. NG2-proteoglycan-dependent contributions of oligodendrocyte progenitors and myeloid cells to myelin damage and repair. Journal of neuroinflammation, 12(1), 161.
Kim, H., et al. 2019. Generation of human pluripotent stem cell-derived fused organoids with oligodendroglia and myelin. STAR protocols, 2(2), p.100443.
Kim, H., et al. 2019. Pluripotent Stem Cell-derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins. Stem cell reports, 12(5), pp.890-905.
Colón, J.M., et al. 2018. Continuous tamoxifen delivery improves locomotor recovery 6 h after spinal cord injury by neuronal and glial mechanisms in male rats. Exp Neurol. 2018 Jan;299(Pt A):109-121.
