Anti-Npas4 Antibody (N408/79)

Name: Anti-Npas4 Antibody
Brand: NeuroMab
SKU: 75-396
Price: 319.0 USD
Availability: InStock

Our Anti-Npas4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N408/79. It detects mouse and rat Npas4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Hippocampus Immunohistochemistry from adult rats treated with kainic acid.

Immunoblot against crude brain low-speed pellets from untreated adult rats (RBLSP), adult rats treated with kainic acid to induce seizures (RBLSP seiz) and adult rats treated with saline (RBLSP non-seiz) probed with N408/79 (left) and N52A/42 (right) TC supe and with Rabbit anti-c-Fos (middle).

Hippocampus Immunohistochemistry from adult rats treated with kainic acid.

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-396

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Npas4

Target Description

Neuronal PAS Domain Protein 4 is encoded by the gene NPAS4. NPAS4 is a transcription factor that is involved in a wide range of physiologic and developmental events. NPAS4 is specifically expressed in neurons. No diseases were found to be associated with NPAS4

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N408/79

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Npas4 Nxf

Molecular Weight

90 kDa

Antigen

Fusion protein amino acids 597-802 (C-terminus) of rat Npas4 (accession number Q8CJH6) produced recombinantly in E. Coli

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2491085

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Neuronal PAS domain-containing protein 4 (Neuronal PAS4) (HLH-PAS transcription factor NXF)

UniProt Details

UniProt (Human): Q8IUM7
UniProt (Immunogen Species): Q8CJH6

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
38958450	1:1000	Wang, N., et al. 2024. Brain regions differences in amyloid-β and gene expression in early APP/PS1 mice and identification of Npas4 as a key molecule in Alzheimer's disease. Biomolecules & Biomedicine, .

Western Blot: Mouse
PMID	Dilution	Publication
38958450	1:1000	Wang, N, et al. 2024. Brain regions differences in amyloid-β and gene expression in early APP/PS1 mice and identification of Npas4 as a key molecule in Alzheimer's disease. Biomolecules & Biomedicine, .

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Anti-Npas4 Antibody (N408/79)

SKU: 75-396

Product Details

Product Specific References for Applications and Species

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