Anti-NMDA NR1 Subunit, Splice Variant N1 Antibody

Name: Anti-NMDA NR1 Subunit, Splice Variant N1 Antibody
Brand: PhosphoSolutions
SKU: 1504-N1
Price: 456.0 USD
Availability: InStock

Our Anti-NMDA NR1 Subunit, Splice Variant N1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR1 Subunit, Splice Variant N1 and is antigen affinity purified. It is great for use in WB, IHC.

Western blot of 10 µg of HEK 293 cells showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the N1 splice variant insert (lane 1). 1) NR1 subunit containing the N1 and C2' Insert; 2) NR1 subunit containing only the C2 Insert; 3) NR1 subunit containing the C1 and C2' Insert; 4) without NR1 expression.

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Species Reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host Species

Rabbit

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Data Sheet

Pooled Serum

SKU: 1504-N1

Size: 25 µg

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

NMDA NR1 Subunit, Splice Variant N1

Scientific Background

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Host Species

Rabbit

Gene Name

GRIN1

Molecular Weight

120 kDa

Immunogen

Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Expected Reactivity

Canine, Chicken, Human, Non-Human Primate, Xenopus

Antibody Registry ID

AB_2492169

Storage

Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Lyophilized

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Buffer

Lyophilized

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000 - 1:2000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~120 kDa NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

GluN1; Glutamate [NMDA receptor subunit zeta-1; Glutamate receptor ionotropic N methyl D aspartate 1; Glutamate receptor ionotropic N-methyl-D aspartate subunit 1; glutamate receptor ionotropic NMDA 1; Grin1; MRD8; N methyl D aspartate receptor; N methyl D aspartate receptor channel subunit zeta 1; N methyl D aspartate receptor subunit NR1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1; NMDA 1; NMDA R1; NMDAR1; NMDA receptor 1; NMDA1; NMDAR; NMDZ1_HUMAN; NR1

UniProt ID

P35439
UniProt Summary: Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:21376300, PubMed:26875626, PubMed:26919761, PubMed:28126851, PubMed:28228639, PubMed:36959261, PubMed:7679115, PubMed:7681588, PubMed:7685113). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP) (PubMed:26875626). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:21376300, PubMed:26875626, PubMed:26919761, PubMed:27164704, PubMed:28095420, PubMed:28105280, PubMed:28126851, PubMed:28228639, PubMed:36959261, PubMed:38538865, PubMed:7679115, PubMed:7681588, PubMed:7685113). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium (By similarity). Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:26875626, PubMed:26919761, PubMed:36309015, PubMed:38598639). {ECO:0000250|UniProtKB:P35438, ECO:0000269|PubMed:21376300, ECO:0000269|PubMed:26875626, ECO:0000269|PubMed:26919761, ECO:0000269|PubMed:27164704, ECO:0000269|PubMed:28095420, ECO:0000269|PubMed:28105280, ECO:0000269|PubMed:28126851, ECO:0000269|PubMed:28228639, ECO:0000269|PubMed:36309015, ECO:0000269|PubMed:36959261, ECO:0000269|PubMed:38538865, ECO:0000269|PubMed:38598639, ECO:0000269|PubMed:7679115, ECO:0000269|PubMed:7681588, ECO:0000269|PubMed:7685113}.

NCBI Gene ID

24408

Shipping

Blue Ice

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Anti-NMDA NR1 Subunit, Splice Variant N1 Antibody

SKU: 1504-N1

Product Details

Bulk Order Anti-NMDA NR1 Subunit, Splice Variant N1 Antibody

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