Anti-Nicastrin, N-terminal Antibody

Name: Anti-Nicastrin, N-terminal Antibody
Brand: Biosensis
SKU: R-1686-500
Price: 337.0 USD
Availability: InStock

Our Anti-Nicastrin, N-terminal rabbit polyclonal primary antibody detects human Nicastrin, N-terminal, and is IgG. It is validated for use in ICC, WB.

Western Immunoblotting with nicastrin N-terminal domain antibody (Catalog Number R-1686-500) of mouse and human Nicastrin protein in embryonic mouse brain and a human cell line (SHSY5Y). Membrane proteins were prepared and loaded as 20 µg protein per lane. Immunizing peptide absorption removed antibody reactivity.

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Species Reactivity

Human

Applications

IP, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1686-500

Volume/Size: 500 µg

Variant

Price:

$337.00

Quantity:

Product Details

Target

Nicastrin, N-terminal

Scientific Background

Nicastrin, a type 1 membrane glycoprotein, is an essential component of the gamma secretase complex which is critical for the cleavage of the amyloid precursor protein and other membrane proteins. Nicastrin is widely expressed in different tissue types. This antibody detects all processed forms of Nicastrin.

Format

IgG

Clonality

Polyclonal

Isotype

IgG

Applications

IP, WB

Host Species

Rabbit

Molecular Weight

145 kDa (See application details.)

Immunogen

A synthetic peptide (RGNSVERKIYIPL-C) corresponding to human Nicastrin [32-44] in the N-terminal region conjugated via additional C-terminal Cys to Diphtheria toxoid.

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.

Physical State

Lyophilized

Production Notes

Protein G purified IgG

Buffer

Lyophilized from PBS, pH 7.4. Contains no preservative.

Dilution Ranges

WB: 3-10 µg/mL

Application Details

Human or mouse brain samples commonly prepared with reducing (50 mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Unprocessed full length human Nicastrin is 709 amino acids, however this protein contains an N-terminal signal peptide which is considered to undergo cleavage during processing and transit to the cell plasma membrane, in addition the protein undergoes glycosylation to produce a glycoprotein of about 145 kDa apparent MW by SDS PAGE.

Conjugate

Unconjugated

Specificity

Confirmed by WB using peptide absorption.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

UniProt ID

Q92542

Shipping

25°C (ambient)

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Anti-Nicastrin, N-terminal Antibody

SKU: R-1686-500

Product Details

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