Anti-Neuropilin-1 (a1 CUB Domain) Antibody (M534)

Name: Anti-Neuropilin-1 (a1 CUB Domain) Antibody
Brand: PhosphoSolutions
SKU: NM5341
Price: 348.0 USD
Availability: InStock

Our Neuropilin-1 (a1 CUB Domain) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Neuropilin-1 (a1 CUB Domain) and is Protein G purified. It is great for use in WB, ICC, IHC.

Western blot image of recombinant human Neuropilin-1 (lanes 1 & 3) and human PC3 cells (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-Neuropilin-1 (NP2111) (lanes 1 & 2) or with mouse monoclonal anti-Neuropilin-1 (lanes 3 & 4).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

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Data Sheet

SKU: NM5341

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Neuropilin-1 (a1 CUB Domain)

Scientific Background

Neuropilins are transmembrane proteins that contain two CUB domains (a1 and a2), two coagulation factor-like domains (b1 and b2), and a MAM domain in the extracellular region. These proteins have short cytoplasmic domains that include a PDZ-binding motif. The neuropilin (NRP) family includes NRP-1, NRP-2A, and NRP-2B. NRP-1 has been implicated as a receptor involved in axon guidance and VEGF signaling. NRP-1 mediates activation of intracellular signaling pathways through interaction with its co-receptors, Plexin-A1 and VEGFRs. The expression of NRP-1, along with the co-receptor Plexin-A1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M534

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

NRP1

Molecular Weight

130

Immunogen

Clone M534 was generated from a neuropilin-1 synthetic peptide (coupled to carrier protein) corresponding to amino acids within the a1 CUB domain of human neuropilin-1. This sequence is highly conserved in rat and mouse neuropilin-1, and has low homology to neuropilin-2.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:250
IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of neuropilin-1 on SDS-PAGE immunoblots of human recombinant neuropilin-1 and in human PC3 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

BDCA4; NRP1; NP1; NRP; VEGF165R; neuropilin-1; transmembrane receptor; VEGF 165; VEGFR

UniProt ID

O14786
UniProt Summary: Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR.

NCBI Gene ID

8829

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Anti-Neuropilin-1 (a1 CUB Domain) Antibody (M534)

SKU: NM5341

Product Details

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