Our Anti-Neuropeptide Y mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L115/13. It detects human, mouse, and rat Neuropeptide Y, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC.
Array tomography immunofluorescence of Lowicryl-embedded sections from adult mouse cortex layer 2/3 with L115/13 (green), GABA (red) and DAPI (blue). Volume reconstruction of 35 serial 70 nm sections show two NPY-positive neurons with varying levels of GABA reactivity, as well as some NPY-reactive axonal varicosities. Images courtesy of Kristina Micheva (Stanford).
Neuropeptide Y is encoded by the gene NPY. NPY is a member of the NPY family. NPY is a neuropeptide that influences many physiological processes including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. NPY functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. NPY is widely expressed in the central nervous system. Diseases associated with NPY include Eating Disorder and Adjustment Disorder.
Purified by Protein A chromatography
Fusion protein amino acids 28-97 (all but signal peptide) of human NPY (accession number P01303) produced recombinantly in E. Coli
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
No cross-reactivity reported
Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
24 months from date of receipt
Shipped on ice packs
Pro-neuropeptide Y [Cleaved into: Neuropeptide Y (Neuropeptide tyrosine) (NPY); C-flanking peptide of NPY (CPON)]