Anti-Neuron specific enolase (NSE) Antibody
Our Anti-Neuron specific enolase (NSE) rabbit polyclonal primary antibody detects human, mouse, and rat Neuron specific enolase (NSE), and is whole serum. It is validated for use in ICC, WB.
![Left: Immunofluorescent analysis of mixed cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Cells were stained with rabbit antibody to NSE (red, 1:500), and co-stained with chicken antibody to GFAP (C-1373-50, green, 1:5,000). Blue: Hoechst nuclear dye. The NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytes. Right: Western blot analysis of tissue and cell lysates using rabbit antibody to NSE (green, 1:5,000). [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6. A single band at about 47 kDa corresponds to the NSE protein, seen only in extracts containing neurons or neuronal lineage cells.](http://www.antibodiesinc.com/cdn/shop/files/r-1396-50-ihc-wb_120x120.jpg?v=1759274440)
Image shows the human embryonic kidney cells line 293 (Hek293). The red channel shows staining with Rabbit polyclonal antibody to Neuron specific enolase R-1396-50, which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker with Mouse monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1), M-1407-100. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.
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