Our Anti-Neurofilament NF-L primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-L and is total IgY fraction. It is great for use in WB, IHC, ICC.
Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).
Total IgY fraction
Polyclonal
IgY
ICC, IHC, WB
Chicken
NEFL
68 kDa
Preparation of bovine spinal cord NF-L.
Bovine, Chicken, Human, Mouse, Rat
AB_2492162
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Specific for endogenous levels of the ~68 kDa Neurofilament L. Works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. Journal of Neuroscience Research 30:47-62. PMID: 10841809
Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. American Journal of Human Genetics 67:37-46. PMID: 1724473
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