Anti-Neurofilament medium polypeptide (NF-M) Antibody (3H11) (3H11)

Our Anti-Neurofilament medium polypeptide (NF-M) mouse monoclonal primary antibody detects bovine, chicken, horse, human, mouse, pig, and rat Neurofilament medium polypeptide (NF-M), and is IgG. It is validated for use in FC, IF, ICC, IHC, WB.

Image of a neuron and surrounding cells in cerebral culture, derived from adult rats, by Immunofluorescence and Immunocytochemistry. The cells were stained with M-1394-100, Neurofilament medium polypeptide (NF-M), Clone 3H11, Mouse mAb, (green), and co-stained with product R-1379-50, Internexin alpha, Rabbit pAb, (red). Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H (green). The surrounding mitotic neuronal progenitor cells (red) express many other neuronal markers.
Analysis by flow cytometry of Neurofilament medium polypeptide (NF-M), (endogenous) expression in mouse neural progenitor cells differentiated from mES and fixed overnight in 70% ethanol (red curve). PE-labelled goat anti-mouse IgG was used as secondary antibody (Blue curve) and negative control processed with secondary antibody only. Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Left: Analysis by Immunofluorescence and Immunohistochemistry of Neurofilament medium polypeptide (NF-M) expression in rat frontal cortex section. Section was stained with M-1394-100, Neurofilament medium polypeptide (NF-M), Clone 3H11, Mouse mAb, (green, 1:5000), and co-stained with product C-1386-50, Neurofilament heavy polypeptide (NF-H), Chicken pAb (red, 1:5000). IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 um sections, and free-floating sections were stained. The NF-M mouse antibody labels neuronal cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the chicken NF-H antibody stains the network of neuronal axons only. Right: Analysis by western blot of NF-M expression in neuronal tissue lysates using M-1394-100 (green, 1:10,000) with a band at approx 145kDa (rodent NF-M) and 160kDa (bovine NF-M). Lane 1: protein standard; Lane 2: rat spinal cord; Lane 3: mouse spinal cord; Lane 4: cow spinal cord; Lane 5: rat sciatic nerve.
Image of a neuron and surrounding cells in cerebral culture, derived from adult rats, by Immunofluorescence and Immunocytochemistry. The cells were stained with M-1394-100, Neurofilament medium polypeptide (NF-M), Clone 3H11, Mouse mAb, (green), and co-stained with product R-1379-50, Internexin alpha, Rabbit pAb, (red). Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H (green). The surrounding mitotic neuronal progenitor cells (red) express many other neuronal markers.
Image of a neuron and surrounding cells in cerebral culture, derived from adult rats, by Immunofluorescence and Immunocytochemistry. The cells were stained with M-1394-100, Neurofilament medium polypeptide (NF-M), Clone 3H11, Mouse mAb, (green), and co-stained with product R-1379-50, Internexin alpha, Rabbit pAb, (red). Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H (green). The surrounding mitotic neuronal progenitor cells (red) express many other neuronal markers.
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Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
Flow, ICC, IF, IHC, WB
Mouse

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Data Sheet

SKU: M-1394-100

Volume/Size: 100 µg
Price:
$327.00

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