Anti-Neurofilament light polypeptide (NF-L) Antibody (DA2) (DA2)

Our Anti-Neurofilament light polypeptide (NF-L) mouse monoclonal primary antibody detects bovine, horse, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is IgG. It is validated for use in FC, IF, ICC, IHC, WB.

Image of a neuron in cell culture, derived from adult rat cortex, by Immunofluorescence and Immunohistochemistry. The large middle cell is stained with M-1391-50, Neurofilament light polypeptide (NF-L), Clone DA2, Mouse mAb, (green), and co-stained with product R-1379-50, Alpha-internexin, Rabbit pAb, (red). The culture was grown under conditions to induce neuronal survival and differentiation, and product R-1379-50 highlights a network of small neurons at early stages of differentiation.
Analysis by western blot of NF-L expression in different tissue lysates with M-1391-50, Neurofilament light polypeptide (NF-L), Clone DA2, Mouse mAb, (green, 1:5,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: protein standard (red); Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord.
Left: Image of a rat frontal cortex section by Immunofluorescence and Immunohistochemistry. The section was stained with M-1391-50, Neurofilament light polypeptide (NF-L), Clone DA2, Mouse mAb, (red, 1:500), and co-stained with product C-1373-50, Glial fibrillary acidic protein (GFAP), Chicken pAb, (green, 1:5,000). IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 um sections, and free-floating section was stained. The NF-L antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the GFAP antibody stains the network of glial cells. Right: Analysis of Neurofilament light polypeptide (NF-L), expression in human prostate cancer cell line DU145 by flow cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to Neurofilament Light (cat # M-1391-50, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Image of a neuron in cell culture, derived from adult rat cortex, by Immunofluorescence and Immunohistochemistry. The large middle cell is stained with M-1391-50, Neurofilament light polypeptide (NF-L), Clone DA2, Mouse mAb, (green), and co-stained with product R-1379-50, Alpha-internexin, Rabbit pAb, (red). The culture was grown under conditions to induce neuronal survival and differentiation, and product R-1379-50 highlights a network of small neurons at early stages of differentiation.
Image of a neuron in cell culture, derived from adult rat cortex, by Immunofluorescence and Immunohistochemistry. The large middle cell is stained with M-1391-50, Neurofilament light polypeptide (NF-L), Clone DA2, Mouse mAb, (green), and co-stained with product R-1379-50, Alpha-internexin, Rabbit pAb, (red). The culture was grown under conditions to induce neuronal survival and differentiation, and product R-1379-50 highlights a network of small neurons at early stages of differentiation.
Click on image to zoom

Bovine, Horse, Human, Mouse, Pig, Rat
Flow, ICC, IF, IHC, WB
Mouse

Go to Full Product Details

Data Sheet

SKU: M-1391-50

Volume/Size: 50 µg
Price:
$327.00

Bulk Order Anti-Neurofilament light polypeptide (NF-L) Antibody (DA2)

Related Products

Recently Viewed