Anti-Neurofilament light polypeptide (NF-L) Antibody (6H112) (6H112)

Our Anti-Neurofilament light polypeptide (NF-L) mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is IgG. It is validated for use in IF, ICC, IHC, WB.

Image of a rat frontal cortex by Immunofluorescence and Immunohistochemistry. The section was stained with M-2114-50, Neurofilament light polypeptide (NF-L), Clone 6H112, Mouse mAb, (green, 1:2000), and co-stained with product R-3771-100, FOX3/NeuN, Rabbit pAb, (red, 1:2,000). Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 µm sections, and free-floating sections were stained. The NF-L antibody labels cell bodies and processes of pyramidal neurons,dendrites, and axons of other neuronal cells. The FOX3/NeuN antibody selectively stains nuclei and cytoplasm of neuronal cells.
Analysis by western blot of NF-L expression in spinal cord lysates from different species with M-2114-50, Neurofilament light polypeptide (NF-L), Clone 6H112, Mouse mAb, (green, 1:5,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: protein standard (red); Lane 2: rat; Lane 3: mouse; Lane 4: pig; Lane 5: cow.
Image of a rat frontal cortex by Immunofluorescence and Immunohistochemistry. The section was stained with M-2114-50, Neurofilament light polypeptide (NF-L), Clone 6H112, Mouse mAb, (green, 1:2000), and co-stained with product R-3771-100, FOX3/NeuN, Rabbit pAb, (red, 1:2,000). Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 µm sections, and free-floating sections were stained. The NF-L antibody labels cell bodies and processes of pyramidal neurons,dendrites, and axons of other neuronal cells. The FOX3/NeuN antibody selectively stains nuclei and cytoplasm of neuronal cells.
Image of a rat frontal cortex by Immunofluorescence and Immunohistochemistry. The section was stained with M-2114-50, Neurofilament light polypeptide (NF-L), Clone 6H112, Mouse mAb, (green, 1:2000), and co-stained with product R-3771-100, FOX3/NeuN, Rabbit pAb, (red, 1:2,000). Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 µm sections, and free-floating sections were stained. The NF-L antibody labels cell bodies and processes of pyramidal neurons,dendrites, and axons of other neuronal cells. The FOX3/NeuN antibody selectively stains nuclei and cytoplasm of neuronal cells.
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Bovine, Human, Mouse, Pig, Rat
ICC, IF, IHC, WB
Mouse

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Data Sheet

SKU: M-2114-50

Volume/Size: 50 µg
Price:
$327.00

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