Anti-Nestin Antibody

Our Anti-Nestin rabbit polyclonal primary antibody detects human Nestin, and is whole serum. It is validated for use in ICC, WB.

Left: SH-SY5Y neuroblastoma cells stained with rabbit antibody to Nestin (red, 1:5,000) and chicken antibody to Vimentin (C-1409-50, green, 1:10,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in SH-SY5Y cell lysate. The antibody (1:2,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.
Left: SH-SY5Y neuroblastoma cells stained with rabbit antibody to Nestin (red, 1:5,000) and chicken antibody to Vimentin (C-1409-50, green, 1:10,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in SH-SY5Y cell lysate. The antibody (1:2,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.
Left: SH-SY5Y neuroblastoma cells stained with rabbit antibody to Nestin (red, 1:5,000) and chicken antibody to Vimentin (C-1409-50, green, 1:10,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in SH-SY5Y cell lysate. The antibody (1:2,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.
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Human
ICC, WB
Rabbit

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Data Sheet

SKU: R-1811-100

Volume/Size: 100 µg
Price:
$327.00

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