Anti-Nestin Antibody

Name: Anti-Nestin Antibody
Brand: Aves Labs
SKU: NES-0020
Price: 481.0 USD
Availability: InStock

Three antipeptide antibodies were generated in chickens against a sequence in mouse (NP_057910) gene product. Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.

Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. Red shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (Aves Labs, 1:500). Green is staining of the granule cells; blue is DAPI nuclear staining. Page Balich, Univ. Arizona.

Photomicrograph of 3T3 cells in culture. Nestin immunoreactivity (red staining, 1:1000 dilution); green is β-Tubulin 3 staining using a rabbit antibody (1:500); blue is DAPI nuclear staining. Page Balich, Univ. Arizona.

Immunocytochemistry showing nestin immunoreactivity (green; 1:1000 dilution) in A7r5 neuroblastoma cells in culture. Blue is DAPI nuclear staining. Page Balich, Univ. Arizona.

Photomicrograph of A7r5 neuroblastoma cells in culture. Nestin immunoreactivity (red staining, Aves Labs, 1:1000 dilution; green is β-Tubulin 3 staining using a rabbit antibody (Rockland, 1:500 dilution); blue is DAPI nuclear staining. Page Balich, Univ. Arizona.

Photomicrograph of a tissue section through the periventricular zone of an e16 mouse brain (4.0% paraformaldehyde-fixed). Green is nestin immunoreactivity (1:1000 dilution). Blue is DAPI nuclear staining. Hoda Ilias, Aves Labs.

Nestin Western Blot. 20 ug of total protein from adult mouse brain in each lane. Lane A is Aves Labs' antibody (1:2000), showing a single, strong band at the correct molecular weight. Lane B uses a rabbit antibody from BD Transduction Labs showing a weak band at the same molecular weight, as well as another spurious band at about 145 kDa. Hoda Ilias, Aves Labs.

Coronal sections of E12.5 p130Cas::BacEGFP cortices stained for the RGC marker Nestin (Cat. NES, 1:500; magenta) and EGFP (green). Counterstain is DAPI (blue). Image from publication CC-BY-4.0. PMID:37540708

Coronal sections of E15.5 p130Cas::BacEGFP cortices stained for the RGC marker Nestin (Cat. NES, 1:500; magenta) and EGFP (green). Counterstain is DAPI (blue). Image from publication CC-BY-4.0. PMID:37540708

Laminin (green) and Nestin (NES, 1:500; magenta) antibody staining on E15.5 control and Emx1Cre;CasTcKO coronal sections. Radial glial endfeet do not make proper contact with the basal lamina in Emx1Cre;CasTcKO animals at exposed Laminin sites (arrows). Image from publication CC-BY-4.0. PMID:37540708

Immunofluorescence for pY165Cas (cyan), c-Myc (magenta), and Nestin (Cat. NES, 1:500; blue) of WT and Emx1Cre;β1-Integrinflox/flox mixed cortical cultures transfected with a control vector (myc) or a construct overexpressing myc-tagged full-length Dag1 (myc-Dag1). Counterstain is DAPI (yellow). Image from publication CC-BY-4.0. PMID:37540708

Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. Red shows nestin immunoreactivity (1:1000) as visualized with a Texas Red goat anti-chicken IgY antibody (Aves Labs, 1:500). Green is staining of the granule cells; blue is DAPI nuclear staining. Page Balich, Univ. Arizona.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Go to Full Product Details

Data Sheet

Trial Size

SKU: NES-0020

Volume: 100 µL

Variant

Price:

$144.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Nestin

Scientific Background

Mouse Nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.

Format

Affinity-Purified IgY

Concentration

300 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

NES Nbla00170

Molecular Weight

250 kDa

Immunogen

Synthetic peptides

Immunogen Species

Human

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2314882

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the mouse nestin gene product (NM_NP_057910), but are shared with the rat (NM_012987, NCBI) protein sequence. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filtersterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:10000-1:20000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Synonyms

ESTM 46; Nestin; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; nes; NEST_HUMAN; Nestin

UniProt ID

Q6P5H2
UniProt Summary: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

NCBI Gene ID

18008

Shipping

Ice packs

Recover password

Create my account

Anti-Nestin Antibody

SKU: NES-0020

Product Details

Bulk Order Anti-Nestin Antibody

Related Products

Recently Viewed