Anti-Nestin Antibody

Our Anti-Nestin chicken polyclonal primary antibody detects human and rat Nestin, and is IgY preparation. It is validated for use in ICC, WB.

Left: SH-SY5Y neuroblastoma cells stained with chicken antibody to Nestin (green, 1:2,000) and rabbit antibody to Vimentin (R-1699-100, red, 1:2,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in rat C6 (Lane 1) and human SH-SY5Y (Lane 2) cell lysates. The antibody (1:1,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.
Nestin staining (red) in human mesenchymal stem cells by Immunocytochemistry. Primary antibody dilution: 1:3,000. Image courtesy of QBM Cell Science.
Nestin staining (red) in normal human atrial cardiac fibroblasts (3DIV) by Immunocytochemistry, using chicken antibody to nestin, C-1812-50. Green: beta-tubulin (left), anti-fibroblast antibody (right). Blue: cell nuclei. Image courtesy of QBM Cell Science.
Left: SH-SY5Y neuroblastoma cells stained with chicken antibody to Nestin (green, 1:2,000) and rabbit antibody to Vimentin (R-1699-100, red, 1:2,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in rat C6 (Lane 1) and human SH-SY5Y (Lane 2) cell lysates. The antibody (1:1,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.
Left: SH-SY5Y neuroblastoma cells stained with chicken antibody to Nestin (green, 1:2,000) and rabbit antibody to Vimentin (R-1699-100, red, 1:2,000) by Immunocytochemistry. Vimentin is main partner of nestin forming heterodimers, further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells is shown in yellow. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of nestin expression in rat C6 (Lane 1) and human SH-SY5Y (Lane 2) cell lysates. The antibody (1:1,000) detects one specific band running at ~260 kDa corresponding to full-length nestin protein.
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Human, Rat
ICC, WB
Chicken

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Data Sheet

SKU: C-1812-50

Volume/Size: 50 µL
Price:
$327.00

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