Anti-NCKX4 Antibody (N414/25)

9 Citations

Our Anti-NCKX4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N414/25. It is KO validated, detects human and rat NCKX4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against membranes from wild- type (N4+/+) or NKCX4 knockout (N4-/-) mouse brains probed with N414/25 TC supe. Data courtesy of Sunita Sharma and Jonathan Lytton (University of Calgary).
Adult rat hippocampus immunohistochemistry.
Immunoblot against extracts of COS cells transiently transfected with Flag- tagged NCKX2, NCKX3 or NCKX4 plasmid and probed with N414/25 (left) or N52A/42 (right) TC supe.
Adult rat brain membrane immunoblot.
Immunostaining of NCKX4 (cat. 75-404; red) along the trajectory of ameloblast differentiation, from dental epithelial stem cells residing in the cervical loop, secretory, transition, to ruffle-ended and smooth-ended maturation ameloblasts, fully coexisting in the continuously growing mouse incisors. Nuclei are labeled with DAPI (blue). Image from publication CC-BY-4.0. PMID:36814474
Immunoblot against membranes from wild- type (N4+/+) or NKCX4 knockout (N4-/-) mouse brains probed with N414/25 TC supe. Data courtesy of Sunita Sharma and Jonathan Lytton (University of Calgary).
Immunoblot against membranes from wild- type (N4+/+) or NKCX4 knockout (N4-/-) mouse brains probed with N414/25 TC supe. Data courtesy of Sunita Sharma and Jonathan Lytton (University of Calgary).
Adult rat hippocampus immunohistochemistry.
Immunoblot against extracts of COS cells transiently transfected with Flag- tagged NCKX2, NCKX3 or NCKX4 plasmid and probed with N414/25 (left) or N52A/42 (right) TC supe.
Adult rat brain membrane immunoblot.
Immunostaining of NCKX4 (cat. 75-404; red) along the trajectory of ameloblast differentiation, from dental epithelial stem cells residing in the cervical loop, secretory, transition, to ruffle-ended and smooth-ended maturation ameloblasts, fully coexisting in the continuously growing mouse incisors. Nuclei are labeled with DAPI (blue). Image from publication CC-BY-4.0. PMID:36814474

Human, Mouse, Rat, Zebrafish
ICC, IHC, WB
Mouse
490
550
594
650

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Data Sheet
KO Validated KO Validated

SKU: 75-404

Volume: 100 µL
1-2 business days
Price:
$319.00

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PMID Dilution Publication
36814474not listedChan, B., et al. 2023. Sodium/(calcium + potassium) Exchanger NCKX4 Optimizes KLK4 Activity in the Enamel Matrix Microenvironment to Regulate ECM Modeling. Frontiers in Physiology, 1116091.
331993721 : 500Thibodeau, S., et al. 2020. Calmodulin binds and modulates K +-dependent Na +/Ca 2+-exchanger isoform 4, NCKX4. Journal of Biological Chemistry, 100092.
29209227not listedJalali, R., et al. 2017. The Role of Na:K:2Cl Cotransporter 1 (NKCC1/SLC12A2) in Dental Epithelium during Enamel Formation in Mice. Frontiers in Physiology, 924.
27752731not listedBronckers, A.L., et al. 2017. Reduced Protein Expression of the Na+/Ca2++K+-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.. Calcified Tissue International, 80-86.
Immunohistochemistry: Zebrafish
PMID Dilution Publication
28650316not listedVinberg, F., et al. 2017. The Na+/Ca2+, K+ exchanger NCKX4 is required for efficient cone-mediated vision.. Elife, e24550.
Western Blot: Mouse
PMID Dilution Publication
331993721 : 500Thibodeau, S., et al. 2020. Calmodulin binds and modulates K +-dependent Na +/Ca 2+-exchanger isoform 4, NCKX4. Journal of Biological Chemistry, 100092.
29209227not listedJalali, R., et al. 2017. The Role of Na:K:2Cl Cotransporter 1 (NKCC1/SLC12A2) in Dental Epithelium during Enamel Formation in Mice. Frontiers in Physiology, 924.
27752731not listedBronckers, A.L., et al. 2017. Reduced Protein Expression of the Na+/Ca2++K+-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.. Calcified Tissue International, 80-86.
Western Blot: Zebrafish
PMID Dilution Publication
28650316not listedVinberg, F., et al. 2017. The Na+/Ca2+, K+ exchanger NCKX4 is required for efficient cone-mediated vision.. Elife, e24550.

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