Our unphosphorylated N-WASP (Ser-484/Ser-485) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat unphosphorylated N-WASP (Ser-484/Ser-485) and is antigen affinity purified. It is great for use in WB, ICC.
Western blot of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 µg/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).
Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
WASL
65
Unphosphorylated N-WASP synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:50
Unconjugated
This antibody was cross-adsorbed to phospho-N-WASP (S484/S485) peptide (without carrier) then affinity purified using unphosphorylated N-WASP (S484/S485) peptide (without carrier). The antibody detects a 65 kDa* protein on SDS-PAGE immunoblots of rat brain and SKN-SH cell lysates that are treated with alkaline phosphatase. Only low levels of unphosphorylated WASP or N-WASP are detected in untreated rat brain, Jurkat, and A431 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Neural Wiskott-Aldrich syndrome protein, WASL, WASP
