Anti-N-Cadherin (C-terminal region) Antibody

Name: Anti-N-Cadherin (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CP1901
Price: 275.0 USD
Availability: InStock

Our N-Cadherin (C-terminal) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat N-Cadherin (C-terminal) and is antigen affinity purified. It is great for use in WB.

Western blot image of human endothelial cells untreated or treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-N-Cadherin (Cytoplasmic), anti-N-Cadherin (Tyr-820), anti-N-Cadherin (a.a. 853-864), and anti-N-Cadherin (Tyr-860).

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: CP1901

Volume: 100 µL

Variant

Price:

$275.00

Quantity:

Product Details

Target

N-Cadherin (C-terminal)

Target Description

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

CDH2

Molecular Weight

130

Antigen

N-Cadherin synthetic peptide (coupled to carrier protein) corresponding to amino acids from the C-terminal region of human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, as well as in E-, P-, and R-cadherin from human, rat, and mouse.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

In western blots, the antibody detects a 130 kDa* band corresponding to N-cadherin in human endothelial cells and detects a 120 kDa band corresponding to E-cadherin in human A431 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Cadherin-2, Neural-Cadherin, CD325

UniProt Details

UniProt (Human): P19022
UniProt (Immunogen Species): P19022

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Anti-N-Cadherin (C-terminal region) Antibody

SKU: CP1901

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