Anti-Myosin Light Chain (N-terminal region) Antibody

Name: Anti-Myosin Light Chain (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: MP4201
Price: 275.0 USD
Availability: InStock

2 Citations

Our Myosin Light Chain (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Myosin Light Chain (N-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.

Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8) .

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: MP4201

Volume: 100 µL

Variant

Price:

$275.00

Quantity:

Product Details

Target

Myosin Light Chain (N-terminal region)

Target Description

Both smooth muscle and nonmuscle myosin II activity is regulated by the phosphorylation state of the myosin regulatory light chain (MLC, MRLC, MLC20, Myl9). Phosphorylation of MLC at Thr-18 and Ser-19 activates myosin II motor activity and increases myosin filament stability. This activation has important roles in various cell motile processes. By contrast, other phosphorylation sites on MLC may inhibit myosin II activity. PKC phosphorylates Ser-1/Ser-2 and Thr-9 in MLC, and this phosphorylation decreases activated myosin II interaction with actin, as well as inhibits MLC interaction with the activation site kinase, myosin light-chain kinase. The Ser-1/Ser-2 region may be the major inhibitory site since Ser-1 is phosphorylated during PDGF-induced stress fiber disassembly and expression of unphosphorylatable MLC20 at the Ser-1/Ser-2 site suppresses this disassembly. Thus, inhibition of myosin II activity through phosphorylation of Ser-1/Ser-2 may have important roles in growth factor-induced reorganization of actomyosin filaments.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

MYL12A

Molecular Weight

Antigen

MLC synthetic peptide (coupled to KLH) corresponds to amino acid residues in the N-terminus of human myosin regulatory light chain 12A (Myl12A). This peptide sequence is highly conserved in human, rat, mouse, chicken, and zebrafish Myl12A, and in other smooth muscle and non-muscle MLCs.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects a 20 kDa* band corresponding to the molecular weight of myosin light chain in mouse C2C12, rat PC12, and human A431.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

MLC20, RLC-C; Mylc2c; Myl9, MLC2, MRLC1, MYRL2; MLCB; MRCL3; MRLC3; MYL2B; MYL12A, myosin

UniProt Details

UniProt (Human): P19105
UniProt (Immunogen Species): P19105

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
34717967	not listed	Monjotin, N, et al. 2022. Lymphotonic Activity of Ruscus Extract, Hesperidin Methyl Chalcone and Vitamin C in Human Lymphatic Smooth Muscle Cells. Microvascular Research, 104274.

Western Blot: Human
PMID	Dilution	Publication
32187552	not listed	Uzureau, S, et al. 2020. APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin. Cell reports, 3821-3836.

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Anti-Myosin Light Chain (N-terminal region) Antibody

SKU: MP4201

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Myosin Light Chain (N-terminal region) Antibody

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