Our Myosin 7B/MHC14 (Hinge region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Myosin 7B/MHC14 (Hinge region) and is antigen affinity purified. It is great for use in WB, ICC.
Western blot analysis MYH7B in mouse brain (lane 1 & 2) and mouse extraocular muscle (lane 3 & 4). The blot was probed with rabbit polyclonal anti-MYH7B/MHC14 (Hinge region) at 1:500 in absence (lanes 1 & 3) and presence of MYH7B blocking peptide (MX4555; lanes 2 & 4).
Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).
Antigen Affinity Purified
Polyclonal
IgG
ICC, WB
Rabbit
MYH7B
215
A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the hinge region from mouse MYH7B/MHC14. This sequence is highly conserved in rat and human MYH7B, and has less than 50% identity with other MYH family members.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
ICC: 1:300
Unconjugated
The antibody detects a 215 kDa* protein corresponding to the apparent molecular mass of MYH7B on SDS-PAGE immunoblots of mouse extraocular muscle and brain.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
