Anti-Myosin 13/Extraocular Myosin (Hinge region) Antibody

Name: Anti-Myosin 13/Extraocular Myosin (Hinge region) Antibody
Brand: PhosphoSolutions
SKU: MP4571
Price: 285.0 USD
Availability: InStock

1 Citation

Our Myosin 13/Extraocular Myosin (Hinge region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Myosin 13/Extraocular Myosin (Hinge region) and is antigen affinity purified. It is great for use in WB, IHC.

Western blot analysis of MYH13 in mouse extraocular muscle. The blot was probed on the left with anti-MYH13/Extraocular myosin (Hinge region) antibody (MP4571) and on the right with anti-MYH13/Extraocular myosin (C-terminal region) antibody (MP4561).

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Species Reactivity

Human, Mouse, Rat

Applications

IHC, WB

Host Species

Rabbit

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SKU: MP4571

Volume: 100 µL

Variant

Price:

$285.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Myosin 13/Extraocular Myosin (Hinge region)

Scientific Background

Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

MYH13

Molecular Weight

215

Immunogen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the hinge region from mouse MYH13/extraocular myosin. This sequence has 100% identity with rat and human MYH13, and has low homology to other myosin family members.

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:150

Conjugate

Unconjugated

Specificity

The antibody detects a 215 kDa* protein corresponding to the apparent molecular mass of MYH13 on SDS-PAGE immunoblots of mouse extraocular muscle, and does not detect MYHs in mouse diaphragm, gastrocnemius, or brain.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Myh13, myosin heavy chain

UniProt ID

B1AR69

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunohistochemistry: Bat | Rat |Songbird | Toadfish

Immunohistochemistry: Bat
PMID	Dilution	Publication
29165242	1:50	Mead, A, et al. 2017. Fundamental constraints in synchronous muscle limit superfast motor control in vertebrates. Elife, e49425.

Immunohistochemistry: Rat
PMID	Dilution	Publication
29165242	1:50	Mead, A, et al. 2017. Fundamental constraints in synchronous muscle limit superfast motor control in vertebrates. Elife, e49425.

Immunohistochemistry: Songbird
PMID	Dilution	Publication
29165242	1:50	Mead, A, et al. 2017. Fundamental constraints in synchronous muscle limit superfast motor control in vertebrates. Elife, e49425.

Immunohistochemistry: Toadfish
PMID	Dilution	Publication
29165242	1:50	Mead, A, et al. 2017. Fundamental constraints in synchronous muscle limit superfast motor control in vertebrates. Elife, e49425.

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Anti-Myosin 13/Extraocular Myosin (Hinge region) Antibody

SKU: MP4571

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Myosin 13/Extraocular Myosin (Hinge region) Antibody

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