Anti-Myelin Basic Protein (MBP) Antibody
Our Anti-Myelin Basic Protein (MBP) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Myelin Basic Protein (MBP) and is total IgY fraction. It is great for use in WB, IHC, ICC.
SKU: 1120-MBP
Product Details
Myelin Basic Protein (MBP)
Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa.
Total IgY fraction
Polyclonal
IgY
ICC, IHC, WB
Chicken
MBP
18/22 kDa
Three peptide sequences conserved in higher vertebrate MBP proteins
Bovine, Human, Mouse, Rat
AB_2492157
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Total IgY fraction
PBS + 10 mM NaN3.
WB: 1:5000
WB Brain: 1:5000
IHC: 1:500-1:5000
ICC: 1:500-1:2000
WB Brain: 1:5000
IHC: 1:500-1:5000
ICC: 1:500-1:2000
Unconjugated
Specific for endogenous levels of the ~18 kDa & ~22 kDa myelin basic protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
GDB antibody, Golli MBP antibody, Golli MBP myelin basic protein antibody, Hemopoietic MBP antibody, HMBPR antibody, HUGO antibody, MBP antibody, MBP_CAVPO antibody, MBP_HUMAN antibody, MGC99675 antibody, MLD antibody, Myelin A1 protein antibody, Myelin A1 Protein basic antibody, Myelin basic protein antibody, Myelin Deficient antibody, Myelin membrane encephalitogenic protein antibody, OTTHUMP00000163776 antibodym, OTTHUMP00000174387 antibody, OTTHUMP00000174388 antibody, SHI antibody, Shiverer antibody, SP antibody
UniProt (Human): P02686
UniProt (Immunogen Species): P02688
UniProt (Immunogen Species): P02688
24547
Product Specific References for Applications and Species
- Immunohistochemistry: Mouse
Immunohistochemistry: Mouse | ||
PMID | Dilution | Publication |
30771034 | 1:500 | Gatto, R.G., Ye, A.Q., et al. 2019. Detection of axonal degeneration in a mouse model of Huntington's disease: comparison between diffusion tensor imaging and anomalous diffusion metrics. MAGMA, Feb 15. |
30117615 | 1:500 | Gatto, R.G., et al. 2018. In vivo diffusion MRI detects early spinal cord axonal pathology in a mouse model of amyotrophic lateral sclerosis. NMR in Biomedicine, Aug;31(8):e3954. |
29175489 | 1:500 | Gatto, R.G., et al. 2018. Diffusion Tensor Imaging Identifies Presymptomatic Axonal Degeneration in the Spinal Cord of ALS Mice. Brain Research 1679, 45–52. |
Product Specific Protocols
- Western Blot Protocol: Download