Anti-Myelin basic protein, degraded (dMBP) Antibody

Name: Anti-Myelin basic protein, degraded (dMBP) Antibody
Brand: Biosensis
SKU: R-107-100
Price: 327.0 USD
Availability: InStock

Our Anti-Myelin basic protein, degraded (dMBP) rabbit polyclonal primary antibody detects guinea pig, human, and rat dMBP, and is whole serum. It is validated for use in IHC-Frozen.

Immunohistochemistry on human brain with brain trauma (car accident), using rabbit antibody to guinea pig degraded myelin basic protein (68-86): whole serum (R-107-100) at a dilution of 1:2000. Courtesy of Dr. Wei-Ping Gai, The Flinders University of South Australia.

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Species Reactivity

Guinea Pig, Human, Pig, Rat

Applications

IHC

Host Species

Rabbit

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Data Sheet

SKU: R-107-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Myelin basic protein, degraded (dMBP)

Scientific Background

Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

IHC

Host Species

Rabbit

Immunogen

A synthetic peptide (YG SLPQKSQRSQ DENPVV, aa: 68-86) as part of guinea pig MBP protein conjugated to KLH

Immunogen Species

Guinea pig

Species Reactivity

Guinea Pig, Human, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Dilution Ranges

IHC: 1:1000-1:4000

Application Details

IHC, frozen, 4% PFA. Antibody immunostains for MBP in abnormally appearing oligodendrocyte processes and cell bodies in demyelinating areas. This antibody recognizes only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerves. It also stains discrete white matter in the brain of multiple system atrophy.

Conjugate

Unconjugated

Specificity

This antiserum recognizes degraded MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indicates a high level of specificity for this antiserum. This antiserum reacts with human and rat MBP. This antibody is particularly useful in neurodegenerative studies.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Degrading/ degraded Myelin Basic Protein; dMBP

UniProt ID

P25188

Shipping

25°C (ambient)

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Anti-Myelin basic protein, degraded (dMBP) Antibody

SKU: R-107-100

Product Details

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