Anti-Mitochondrial Fission Factor (Mff) Antibody

Name: Anti-Mitochondrial Fission Factor (Mff) Antibody
Brand: PhosphoSolutions
SKU: 1157-MFF
Price: 375.0 USD
Availability: InStock

2 Citations

Our Anti-Mitochondrial Fission Factor (Mff) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects bovine, human, and mouse Mitochondrial Fission Factor (Mff) and is neat serum. It is great for use in WB, ICC.

Western blot of mouse whole brain lysate showing specific immunolabeling of the ~38 kDa Mff protein.

Species Reactivity

Bovine, Human, Mouse

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

SKU: 1157-MFF

Volume: 100 µL

Variant

Price:

$375.00

Quantity:

Product Details

Target

Mitochondrial Fission Factor (MFF)

Target Description

Mitochondrial fission factor, also known as Mff, is a tail-anchored, outer mitochondrial membrane protein that is part of a complex process controlling mitochondrial and peroxisomal fission in conjunction with Drp1 and Fis1 (Schrader and Yoon, 2007). The rate of mitochondrial fission and fusion balance each other for cell growth and survival of mitochondria. Fission can be greatly accelerated when cytochrome c is released during apoptosis (Desagher and Martinou, 2000). Mff was identified as an important component of the process through siRNA transfected cells, isolating the protein in the P2 pellet and demonstrating that Mff is exposed to the cytosol (Gandre-Babbe and van der Bliek 2008). Mff has been identified at different stages of the fission process working alongside, rather than in complex with, Fis1 suggesting that Mff contributes to fission independent of the Fis1 complex (Gandre-Babbe and van der Bliek 2008).

Format

Neat Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

MFF

Molecular Weight

38 kDa

Antigen

Human recombinant protein

Species Reactivity

Bovine, Human, Mouse

Antibody Registry ID

AB_2736925

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Neat sera

Buffer

Neat serum + 0.09% NaN3

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~38 kDa Mff protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

C2orf33 antibody, Chromosome 2 open reading frame 33 antibody, DKFZp666J168 antibody, GL004 antibody, Mff antibody, MFF_HUMAN antibody, MGC110913 antibody, Mitochondrial fission factor antibody, OTTHUMP00000164235 antibody

UniProt Details

UniProt (Human): Q9GZY8
UniProt (Immunogen Species): Q9GZY8

NCBI Gene ID

56947

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
18353969	1:200	Gandre-Babbe, S., et al. 2008. The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells. Molecular Biology of the Cell, 19(6), pp.2402-2412.

Western Blot: Human
PMID	Dilution	Publication
18353969	not listed	Gandre-Babbe, S., et al. 2008. The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells. Molecular Biology of the Cell, 19(6), pp.2402-2412.

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Product Specific Protocols

Western Blot Protocol: Download

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Anti-Mitochondrial Fission Factor (Mff) Antibody

SKU: 1157-MFF

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Mitochondrial Fission Factor (Mff) Antibody

Product Specific Protocols

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