Anti-Mitochondria Antibody (113-1)
Our Anti-Mitochondria primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Mitochondria and is Protein A purified. It is great for use in WB, IHC, ICC.
 and Aves Labs chicken anti-TOMM20 antibody (Cat No. TOMM20). The cells were fixed with 3% paraformaldehyde, washed with PBS, and blocked/permeabilized with 5% non-fat milk powder in PBST. The anti-Mitochondria antibody (Cat No. 1155-MIT) and the anti-TOMM20 antibody were incubated with the sample at a dilution of 1:800 and 1:200, respectively, at 4°C overnight. After washing with PBST, the cells were stained using goat anti-mouse IgY CF488 secondary antibody (Cat No. 80-1001-488) (green) and goat anti-mouse 594 secondary antibody (red). The staining revealed a complete overlap between the signal from the chicken anti-TOMM20 antibody and the mouse anti-Mitochondria antibody specifically in the cell mitochondria only in HeLa cells. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). DAPI nuclear stain (blue) shows cell nuclei.](http://www.antibodiesinc.com/cdn/shop/files/1155-MIT-mitochondria-immunocytochemistry-icc-1_120x120.jpg?v=1790100466)
 and PhosphoSolutions anti-Mitochondria mouse monoclonal antibody [113-1] (Cat No. 1155-MIT). 4-12% Bis-Tris gel was loaded with 20 ug of HeLa and NIH3T3 cell lysates. Proteins were transferred to PVDF membrane and blocked with 5% non-fat milk powder. Anti-Mitochondria chicken (Cat No. 78-607) (lanes 1, 2) and anti-Mitochondria mouse (Cat No. 1155-MIT) (lanes 3, 4) antibodies were incubated with the membrane at a concentration of 1:1000 (1ug/ml). The primary antibodies were then detected via chemiluminescence (6 seconds) using a goat anti-Chicken H+L, HRP conjugated (lanes 1, 2) or goat anti-mouse H+L, HRP conjugated (lanes 3, 4) secondary antibodies. Anti-Mitochondria chicken and mouse antibodies recognize a non-glycosylated protein component of mitochondria only in human HeLa cell lysates and not in mouse NIH3T3 cell lysates at ~60 kDa.](http://www.antibodiesinc.com/cdn/shop/files/1155-MIT-mitochondria-western-blot-wb-2_120x120.jpg?v=1790100466)
 and Aves Labs chicken anti-TOMM20 antibody (Cat No. TOMM20). The cells were fixed with 3% paraformaldehyde, washed with PBS, and blocked/permeabilized with 5% non-fat milk powder in PBST. The anti-Mitochondria antibody (Cat No. 1155-MIT) and the anti-TOMM20 antibody were incubated with the sample at a dilution of 1:800 and 1:200, respectively, at 4°C overnight. After washing with PBST, the cells were stained using goat anti-mouse IgY CF488 secondary antibody (Cat No. 80-1001-488) (green) and goat anti-mouse 594 secondary antibody (red). The staining revealed a complete overlap between the signal from the chicken anti-TOMM20 antibody and the mouse anti-Mitochondria antibody specifically in the cell mitochondria only in HeLa cells. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). DAPI nuclear stain (blue) shows cell nuclei.](http://www.antibodiesinc.com/cdn/shop/files/1155-MIT-mitochondria-immunocytochemistry-icc-1_1600x.jpg?v=1790100466)
Immunofluorescent staining of HeLa and NIH3T3 cells using PhosphoSolutions anti-Mitochondria (human specific) mouse monoclonal antibody [113-1](Cat No. 1155-MIT) and Aves Labs chicken anti-TOMM20 antibody (Cat No. TOMM20). The cells were fixed with 3% paraformaldehyde, washed with PBS, and blocked/permeabilized with 5% non-fat milk powder in PBST. The anti-Mitochondria antibody (Cat No. 1155-MIT) and the anti-TOMM20 antibody were incubated with the sample at a dilution of 1:800 and 1:200, respectively, at 4°C overnight. After washing with PBST, the cells were stained using goat anti-mouse IgY CF488 secondary antibody (Cat No. 80-1001-488) (green) and goat anti-mouse 594 secondary antibody (red). The staining revealed a complete overlap between the signal from the chicken anti-TOMM20 antibody and the mouse anti-Mitochondria antibody specifically in the cell mitochondria only in HeLa cells. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat No. AR-6501). DAPI nuclear stain (blue) shows cell nuclei.
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- Western Blot Protocol: Download