Anti-MFF Antibody (N382/14)

Name: Anti-MFF Antibody
Brand: NeuroMab
SKU: 75-366
Price: 308.0 USD
Availability: InStock

from NeuroMab Datasheet KO Validated

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

N382/14

Applications

ICC, IHC, WB

Host Species

Mouse

Format

Purified

Gene Name

MFF C2orf33 AD030 AD033 GL004

Molecular Weight

40 kDa

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Immunoblot against crude extracts of COS cells transiently transfected with GFP-tagged MFF, MFFDexon1 or untagged Kv2.1 plasmid, adult rat brain membranes (RBM) or MFF wild-type (WT) or knockout (KO) mouse embryonic fibroblast cells probed with N382/14 TC supe (left), rabbit polyclonal (middle) or N52A/42 TC supe (right). Mouse cell samples courtesy of Jodi Nunnari (UC Davis.

Adult rat brain immunohistochemistry.

Volume: 100 µL

Variant

Price:

$308

Quantity:

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Product Details

Target

Mitochondrial fission factor

Target Description

Mitochondrial fission factor, also known as Mff, is a tail-anchored, outer mitochondrial membrane protein that is part of a complex process controlling mitochondrial and peroxisomal fission in conjunction with Drp1 and Fis1 (Schrader and Yoon, 2007). The rate of mitochondrial fission and fusion balance each other for cell growth and survival of mitochondria. Fission can be greatly accelerated when cytochrome c is released during apoptosis (Desagher and Martinou, 2000). Mff was identified as an important component of the process through siRNA transfected cells, isolating the protein in the P2 pellet and demonstrating that Mff is exposed to the cytosol (Gandre-Babbe and van der Bliek 2008). Mff has been identified at different stages of the fission process working alongside, rather than in complex with, Fis1 suggesting that Mff contributes to fission independent of the Fis1 complex (Gandre-Babbe and van der Bliek 2008).

Format

Purified

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N382/14

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

MFF C2orf33 AD030 AD033 GL004

Molecular Weight

40 kDa

Antigen

Fusion protein amino acids 1-173 (MSKRTSSDTPLGRVSGAAFPSPTASEMAEISRIQYEMEYTEGIS QRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQS TPFKPLALKTPPRVLTLSERPLDFLDLERPPVTPQNEEIRAVGRLKRERSMSENAVRQNGQL VRNDSV, cytoplasmic N-terminal exons 1, 2, 3 and 4) and 272-322 (YGISNIEATIEGTSDDM TVVDAASLRRQIIKLNRRLQLLEEENKERAKREM, cytoplasmic N-terminal exons 8 and most of 9) of mostly human MFF (also known as Mitochondrial fission factor, C2orf33, AD030, AD033 and GL004, accession number Q9GZY8); Human: 96% identity (167/173 amino acids identical) and 94% identity (48/51 amino acids identical); Rat: 95% identity (141/147 amino acids identical) and 92% identity (47/51 amino acids identical); Mouse: 93% identity (138/147 amino acids identical) and 84% identity (43/51 amino acids identical)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315891

Storage

Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Buffer

10mM Tris, 50mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Conjugate

Unconjugated

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from opening

Synonyms

Mitochondrial fission factor

UniProt Details

UniProt (Human): Q9GZY8
UniProt (Immunogen Species): Q9GZY8

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Anti-MFF Antibody (N382/14)

Anti-MFF Antibody
SKU: 75-366

Bulk Order Anti-MFF Antibody

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Anti-MFF Antibody (N382/14)

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